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Cytochrome c on Silica Nanoparticles: Influence of Nanoparticle Size on Protein Structure, Stability, and Activity
Abstract
The structure, thermodynamic and kinetic stability, and activity of cytochrome c (cyt c) on silica nanoparticles (SNPs) of different sizes have been studied. Adsorption of cyt c onto larger SNPs results in both greater disruption of the cyt c global structure and more significant changes of the local heme microenvironment than upon adsorption onto smaller SNPs. The disruption of the heme microenvironment leads to a more solvent‐accessible protein active site, as suggested by Soret circular dichroism spectroscopy and through an increase in peroxidase activity as a function of increased SNP size. Similarly, the stability of cyt c decreases more dramatically upon adsorption onto larger SNPs. These results are consistent with changes in protein–nanoparticle interactions that depend on the size or surface curvature of the supporting nanostructure. This study provides further fundamental insights into the effects of nanoscale surfaces on adsorbed protein structure and function.
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Cited by
Monopoli, M. P., Walczyk, D., Campbell, A., Elia, G., Lynch, I., Baldelli Bombelli, F., & Dawson, K. A. (2011) · Journal of the American Chemical Society · doi:10.1021/ja107583h · Cited by 1,835
Walkey, C. D., & Chan, W. C. W. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15233e · Cited by 1,758
Engineered nanoparticles interacting with cells: size matters
Shang, L., Nienhaus, K., & Nienhaus, G. U. (2014) · Journal of Nanobiotechnology · doi:10.1186/1477-3155-12-5 · Cited by 1,316
Schleh, C., Semmler-Behnke, M., Lipka, J., Wenk, A., Hirn, S., Schäffler, M., Schmid, G., Simon, U., & Kreyling, W. G. (2012) · Nanotoxicology · doi:10.3109/17435390.2011.552811 · Cited by 325
Physico-chemical features of engineered nanoparticles relevant to their toxicity
Fubini, B., Ghiazza, M., & Fenoglio, I. (2010) · Nanotoxicology · doi:10.3109/17435390.2010.509519 · Cited by 297
Metadata
- Authors
- Shang, W., Nuffer, J. H., Muñiz‐Papandrea, V. A., Colón, W., Siegel, R. W., & Dordick, J. S.
- Venue
- Small
- Publisher
- Wiley
- Published
- 2009-02-19
- Volume
- 5
- Issue
- 4
- Pages
- 470-476
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2082370264
- Cited by
- 221
- References
- 0
- FWCI
- 5.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shang, W., Nuffer, J. H., Muñiz‐Papandrea, V. A., Colón, W., Siegel, R. W., & Dordick, J. S. (2009). Cytochrome c on Silica Nanoparticles: Influence of Nanoparticle Size on Protein Structure, Stability, and Activity. Small. https://doi.org/10.1002/smll.200800995
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