Cited by 8Hematopoietic Stem Cell TransplantationMesenchymal stem cell researchPluripotent Stem Cells Research

Stem cell defect in ubiquitin-green fluorescent protein mice facilitates engraftment of lymphoid-primed hematopoietic stem cells

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Faltusová, Kateřina, Szikszai, Katarína, Molík, Martin, Linhartová, Jana, Páral, Petr, Šefc, Luděk, Savvulidi, Filipp, Nečas, Emanuel·2018·Stem Cells

Abstract

Transgenic mice expressing green fluorescent protein (GFP) are useful in transplantation experiments. When we used ubiquitin-GFP (UBC-GFP) transgenic mice to study the availability of niches for transplanted hematopoietic stem and progenitor cells, the results were strikingly different from the corresponding experiments that used congenic mice polymorphic in the CD45 antigen. Analysis of these unexpected results revealed that the hematopoiesis of UBC-GFP mice was outcompeted by the hematopoiesis of wild-type (WT) mice. Importantly, UBC-GFP mice engrafted the transplanted bone marrow of WT mice without conditioning. There was a significant bias toward lymphopoiesis in the WT branch of chimeric UBC-GFP/WT hematopoiesis. A fraction of immature Sca-1+ cells in the spleen of UBC-GFP mice expressed GFP at a very high level. The chimeric hematopoiesis was stable in the long term and also after transplantation to secondary recipient mice. The article thus identifies a specific defect in the hematopoiesis of UBC-GFP transgenic mice that compromises the lymphoid-primed hematopoietic stem cells in the bone marrow and spleen.

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Authors
Faltusová, K., Szikszai, K., Molík, M., Linhartová, J., Páral, P., Šefc, L., Savvulidi, F., & Nečas, E.
Affiliations
  • Charles University (CZ)
  • Czech Academy of Sciences (CZ)
Corresponding author
Nečas, Emanuel
Venue
Stem Cells
Published
2018-04-15
Volume
36
Issue
8
Pages
1237-1248
ISSN
  • 1066-5099 (ISSN-L)
  • 1549-4918
OpenAlex
W2794631965
Cited by
8
References
29
Citations by year
2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1
FWCI
0.72
Type
journal article
Language
en
Open access
Locations
  • Stem Cells (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Agency of the Czech Republic (GACR 14-25515S, GACR 17-01897S)
  • Charles University (SVV 260371/2017, UNCE 204021/2012)
  • National Program of Sustainability II (LQ1604)
  • Grant Agency of the Czech Republic (GACR 14‐25515S, GACR 17‐01897S)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
hematopoietic stem cells, hematopoietic engraftment, hematopoietic niche, hematopoietic chimerism, lymphocyte development, Sca-1+ cells, spleen, bone marrow, GFP expression
MeSH terms
Animals, Bone Marrow, Chimera, Hematopoiesis, Hematopoietic Stem Cells, Lymphocytes, Male, Mice, Inbred C57BL, Mice, Transgenic, Spleen, Splenectomy, Thymus Gland, Hematopoietic Stem Cell Transplantation, Ubiquitin, Lymphopoiesis, Green Fluorescent Proteins

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