Stem cell defect in ubiquitin-green fluorescent protein mice facilitates engraftment of lymphoid-primed hematopoietic stem cells
Open accessAbstract
Transgenic mice expressing green fluorescent protein (GFP) are useful in transplantation experiments. When we used ubiquitin-GFP (UBC-GFP) transgenic mice to study the availability of niches for transplanted hematopoietic stem and progenitor cells, the results were strikingly different from the corresponding experiments that used congenic mice polymorphic in the CD45 antigen. Analysis of these unexpected results revealed that the hematopoiesis of UBC-GFP mice was outcompeted by the hematopoiesis of wild-type (WT) mice. Importantly, UBC-GFP mice engrafted the transplanted bone marrow of WT mice without conditioning. There was a significant bias toward lymphopoiesis in the WT branch of chimeric UBC-GFP/WT hematopoiesis. A fraction of immature Sca-1+ cells in the spleen of UBC-GFP mice expressed GFP at a very high level. The chimeric hematopoiesis was stable in the long term and also after transplantation to secondary recipient mice. The article thus identifies a specific defect in the hematopoiesis of UBC-GFP transgenic mice that compromises the lymphoid-primed hematopoietic stem cells in the bone marrow and spleen.
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Control test hematopoietic stem cells possessed an advantage without IFNγ due to the described fitness deficit of Ubc-GFP hematopoietic stem cells.
“Control test HSCs possessed an advantage without IFNγ due to the described fitness deficit of Ubc-GFP HSCs (30).”
From <i>Txnip</i> Enhances Fitness of <i>Dnmt3a</i> -Mutant Hematopoietic Stem Cells via <i>p21</i> (2022) · cites this paper as [30]
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- Authors
- Faltusová, K., Szikszai, K., Molík, M., Linhartová, J., Páral, P., Šefc, L., Savvulidi, F., & Nečas, E.
- Affiliations
- Charles University (CZ)
- Czech Academy of Sciences (CZ)
- Corresponding author
- Nečas, Emanuel
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2018-04-15
- Volume
- 36
- Issue
- 8
- Pages
- 1237-1248
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2794631965
- Cited by
- 8
- References
- 29
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Stem Cells (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agency of the Czech Republic (GACR 14-25515S, GACR 17-01897S)
- Charles University (SVV 260371/2017, UNCE 204021/2012)
- National Program of Sustainability II (LQ1604)
- Grant Agency of the Czech Republic (GACR 14‐25515S, GACR 17‐01897S)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- hematopoietic stem cells, hematopoietic engraftment, hematopoietic niche, hematopoietic chimerism, lymphocyte development, Sca-1+ cells, spleen, bone marrow, GFP expression
- MeSH terms
- Animals, Bone Marrow, Chimera, Hematopoiesis, Hematopoietic Stem Cells, Lymphocytes, Male, Mice, Inbred C57BL, Mice, Transgenic, Spleen, Splenectomy, Thymus Gland, Hematopoietic Stem Cell Transplantation, Ubiquitin, Lymphopoiesis, Green Fluorescent Proteins
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