Protective effects of folic acid on PM2.5-induced cardiac developmental toxicity in zebrafish embryos by targeting AhR and Wnt/β-catenin signal pathways: YUE et al.
Abstract
Our previous observations indicated that extractable organic matter (EOM) from PM2.5 induced malformations in the heart of zebrafish embryos by activating AhR and inhibiting canonical Wnt/β-catenin signal pathway. As a nutritional factor, folic acid (FA) is reported to prevent cardiac defects during embryo development. Hence, we hypothesize that FA may prevent PM2.5-induced heart defects by interfering with AhR and Wnt/β-catenin signaling pathways. Our results showed that FA supplementation alleviated the EOM-induced heart defects in zebrafish embryos, and both AhR inhibitor CH223191 and Wnt activator CHIR99021 enhanced the protective efficiency of FA. Furthermore, FA supplementation attenuated the EOM-induced upregulation of AhR and its target genes including Cyp1a1, Cyp1b1, Ahrra, and Ahrrb. EROD assay confirmed that the EOM agonized Cyp1a1 activity was diminished by FA. The EOM-induced downregulation of β-catenin and its target genes including Nkx2.5, Axin2, Sox9b, and Cox2b were recovered or even overexpressed in embryos exposed to EOM plus FA. In conclusion, our study suggested that FA supplementation protected against PM2.5 cardiac development toxicity by targeting AhR and Wnt/β-catenin signal pathways.
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Folic acid was shown to protect against PM2.5-induced cardiac toxicity in embryos in a developmental zebrafish model.
“In a developmental zebrafish model, folic acid was shown to protect against PM2.5-induced cardiac toxicity in embryos [224].”
From Air pollution and children’s health—a review of adverse effects associated with prenatal exposure from fine to ultrafine particulate matter (2021) · cites this paper as [224]
Cites
Placental DNA hypomethylation in association with particulate air pollution in early life
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Johnson, N. M., Hoffmann, A. R., Behlen, J. C., Lau, C., Pendleton, D., Harvey, N., Shore, R., Li, Y., Chen, J., Tian, Y., et al. (2021) · Environmental Health and Preventive Medicine · doi:10.1186/s12199-021-00995-5 · Cited by 339 · see citing works
“5-induced cardiac toxicity in embryos [224].”
- Authors
- Yue, C., Ji, C., Zhang, H., Zhang, L. W., Tong, J., Jiang, Y., & Chen, T.
- Affiliations
- Soochow University (CN)
- Corresponding author
- Jiang, Yan
- Chen, Tao
- Venue
- Environmental Toxicology
- Publisher
- Wiley
- Published
- 2017-07-19
- Volume
- 32
- Issue
- 10
- Pages
- 2316-2322
- ISSN
- 1520-4081 (ISSN-L)
- OpenAlex
- W2736915537
- Cited by
- 58
- References
- 2
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 9 · 2023: 5 · 2022: 8 · 2021: 9 · 2020: 9 · 2019: 4 · 2018: 4
- FWCI
- 2.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Environmental Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Nature Sciences Foundation of China (81570284, 31300969)
- The Priority Academic Program Development of Jiangsu Higher Education Institutions
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- folic acid, PM2.5, extractable organic matter, zebrafish embryos, AhR signaling pathway, Wnt/β-catenin signaling, CYP1A1, CYP1B1, Nkx2.5, sox9b, CH223191, CHIR99021, air pollution cardiotoxicity
- MeSH terms
- Air Pollutants, Animals, Embryo, Nonmammalian, Folic Acid, Heart, Zebrafish, Signal Transduction, Receptors, Aryl Hydrocarbon, Cytochrome P-450 CYP1A1, Wnt Proteins, beta Catenin, Particulate Matter, Cytochrome P-450 CYP1B1
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