The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Bimolecular fluorescence complementation analysis system for in vivo detection of protein–protein interaction in Saccharomyces cerevisiae
Abstract
The bimolecular fluorescence complementation (BiFC) assay has been widely accepted for studying in vivo detection of protein–protein interactions in several organisms. To facilitate the application of the BiFC assay to yeast research, we have created a series of plasmids that allow single‐step, PCR‐based C‐ or N‐terminal tagging of yeast proteins with yellow fluorescent protein fragments for BiFC assay. By examination of several interacting proteins (Sis1–Sis1, Net1–Sir2, Cet1–Cet1 and Pho2–Pho4), we demonstrate that the BiFC assay can be used to reliably analyse the occurrence and subcellular localization of protein–protein interactions in living yeast cells. The sequences for the described plasmids were submitted to the GenBank under Accession Nos: EF210802, pFA6a‐VN‐His3MX6; EF210803, pFA6a‐VC‐His3MX6; EF210804, pFA6a‐VN‐TRP1; EF210807, pFA6a‐VC‐TRP1; EF210808, pFA6a‐VN‐kanMX6; EF210809, pFA6a‐VC‐kanMX6; EF210810, pFA6a‐His3MX6‐P GAL1‐VN; EF210805, pFA6a‐His3MX6‐P GAL1‐VC; EF210806, pFA6a‐TRP1‐P GAL1‐VN; EF210811, pFA6a‐TRP1‐P GAL1‐VC; EF210812, pFA6a‐kanMX6‐P GAL1‐VN; EF210813, pFA6a‐kanMX6‐P GAL1‐VC; EF521883, pFA6a‐His3MX6‐P CET1‐VN; EF521884, pFA6a‐His3MX6‐P CET1‐VC; EF521885, pFA6a‐TRP1‐P CET1‐VN; EF521886, pFA6a‐TRP1‐P CET1‐VC; EF521887, pFA6a‐kanMX6‐P CET1‐VN; EF521888, pFA6a‐kanMX6‐P CET1‐VC. Copyright © 2007 John Wiley & Sons, Ltd.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · cited 5×
“Considering the fact that cells expressing the C-terminally GFPtagged Cet1 show a normal growth pattern (Huh et al., 2003), it is unlikely that C-terminal protein tagging disturbed the cellular function of Cet1 and caused the failure of BiFC complex formation.”
BackgroundMethodInfluentialSelf-citation
Cited by
Shai, N., Yifrach, E., van Roermund, C. W. T., Cohen, N., Bibi, C., IJlst, L., Cavellini, L., Meurisse, J., Schuster, R., Zada, L., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-03957-8 · Cited by 320
Panchaud, N., Péli-Gulli, M.-P., & De Virgilio, C. (2013) · Science Signaling · doi:10.1126/scisignal.2004112 · Cited by 286
Ha, C. W., & Huh, W.-K. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkq895 · Cited by 77
Self-citationCastro, I. G., Shortill, S. P., Dziurdzik, S. K., Cadou, A., Ganesan, S., Valenti, R., David, Y., Davey, M., Mattes, C., Thomas, F. B., et al. (2022) · eLife · doi:10.7554/elife.74602 · Cited by 60
Metadata
- Authors
- Sung, M., & Huh, W.
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 2007-05-29
- Volume
- 24
- Issue
- 9
- Pages
- 767-775
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W2160360032
- Cited by
- 189
- References
- 1
- FWCI
- 4.60
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
