The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
An expanded tool kit for the auxin‐inducible degron system in budding yeast
Open accessAbstract
Fusion of inducible degradation signals, so‐called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin‐inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR‐based genomic tagging strategies, allow for C‐ or N‐terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., & Kanemaki, M. (2009) · Nature Methods · doi:10.1038/nmeth.1401 · Cited by 1,663
The Elg1 replication factor C-like complex functions in PCNA unloading during DNA replication
Kubota, T., Nishimura, K., Kanemaki, M. T., & Donaldson, A. D. (2013) · Molecular Cell · doi:10.1016/j.molcel.2013.02.012 · Cited by 279
Structural assembly of cullin-RING ubiquitin ligase complexes
Zimmerman, E. S., Schulman, B. A., & Zheng, N. (2010) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2010.08.010 · Cited by 227 · cited 2×
“…catalytic RING finger protein (RBX1), the adaptor SKP1 and an F-box protein as a substrate recognition subunit, is highly conserved among eukaryotes (Zimmerman et al., 2010), such that the plant F-box protein TIR1 is able to form an active E3 with the remaining SCF components from other organisms.”
Background
Cited by
Nora, E. P., Goloborodko, A., Valton, A.-L., Gibcus, J. H., Uebersohn, A., Abdennur, N., Dekker, J., Mirny, L. A., & Bruneau, B. G. (2017) · Cell · doi:10.1016/j.cell.2017.05.004 · Cited by 1,709
Wutz, G., Várnai, C., Nagasaka, K., Cisneros, D. A., Stocsits, R. R., Tang, W., Schoenfelder, S., Jessberger, G., Muhar, M., Hossain, M. J., et al. (2017) · The EMBO Journal · doi:10.15252/embj.201798004 · Cited by 864
Zhang, L., Ward, J. D., Cheng, Z., & Dernburg, A. F. (2015) · Development · doi:10.1242/dev.129635 · Cited by 599
Chemically induced proximity in biology and medicine
Stanton, B. Z., Chory, E. J., & Crabtree, G. R. (2018) · Science · doi:10.1126/science.aao5902 · Cited by 475
Loop extrusion as a mechanism for formation of DNA damage repair foci
Arnould, C., Rocher, V., Finoux, A.-L., Clouaire, T., Li, K., Zhou, F., Caron, P., Mangeot, P. E., Ricci, E. P., Mourad, R., et al. (2021) · Nature · doi:10.1038/s41586-021-03193-z · Cited by 341
Metadata
- Authors
- Morawska, M., & Ulrich, H. D.
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 2013-07-23
- Volume
- 30
- Issue
- 9
- Pages
- 341-351
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W1560496206
- Cited by
- 429
- References
- 3
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: An expanded tool kit for the auxin‐inducible degron system in budding yeast. https://app.cochat.ai/papers/10.1002/yea.2967
- The paper
- Morawska, M., & Ulrich, H. D. (2013). An expanded tool kit for the auxin‐inducible degron system in budding yeast. Yeast. https://doi.org/10.1002/yea.2967
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
