An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Abstract
Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function.
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The AID* tag depletes CTCF with efficiency equivalent to that of the original full-length 231-amino-acid AID tag.
“We observed equivalent CTCF depletion efficiency with the AID* as with the original full-length 231 amino-acid tag (Nishimura et al., 2009) (data not shown).”
From Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization (2017) · cites this paper as Nishimura et al., 2009
The AiD system enables rapid, reversible degradation of essential POIs in cell lines derived from various organisms including chicken, mouse, yeast, and humans.
“The Kanemaki lab demonstrated this possibility with rapid, reversible degradation of essential POIs in cell lines derived from various organisms including chicken, mouse, yeast, and humans [24].”
From Advances in targeted degradation of endogenous proteins (2019) · cites this paper as [24]
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- Authors
- Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., & Kanemaki, M.
- Affiliations
- The University of Osaka (JP)
- National Institute of Genetics (JP)
- Corresponding author
- Kanemaki, Masato
- Venue
- Nature Methods
- Published
- 2009-11-15
- Volume
- 6
- Issue
- 12
- Pages
- 917-922
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W1965813899
- Semantic Scholar
- 875af8df74c6b61ef0e043a074ed2b9f8c8ec48c
- Cited by
- 1,663
- References
- 36
- Citations by year
- 2026: 112 · 2025: 145 · 2024: 178 · 2023: 152 · 2022: 146 · 2021: 168 · 2020: 150 · 2019: 133 · 2018: 107 · 2017: 87 · 2016: 76 · 2015: 64 · 2014: 50 · 2013: 41 · 2012: 30
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Degradation and Inhibitors
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- auxin-inducible degron, protein degradation, auxin, SCF ubiquitin ligase, protein stability, yeast, mammalian cell lines
- MeSH terms
- Animals, Cell Line, Chickens, Indoleacetic Acids, Proteins, Saccharomyces cerevisiae, SKP Cullin F-Box Protein Ligases, Green Fluorescent Proteins
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