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A rapid method for localized mutagenesis of yeast genes
Abstract
We have developed a simple procedure for the localized mutagenesis of yeast genes. In this technique the region of interest is first amplified under mutagenic chain reaction (PCR) conditions. Cotransformation of the PCR product with a gapped plasmid containing homology to both ends the PCR procuct allows in vivo recombination to repair the gap with the mutagenized DNA. This procedure is efficient, allows trageting of specific regions for mutagenesis, and requires no subcloning steps in Escherichia coli.
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Citations
Cited by
Mitochondrial Fusion in Yeast Requires the Transmembrane GTPase Fzo1p
Hermann, G. J., Thatcher, J. W., Mills, J. P., Hales, K. G., Fuller, M. T., Nunnari, J., & Shaw, J. M. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.2.359 · Cited by 549
Many human L1 elements are capable of retrotransposition
Sassaman, D. M., Dombroski, B. A., Moran, J. V., Kimberland, M. L., Naas, T. P., DeBerardinis, R. J., Gabriel, A., Swergold, G. D., & Kazazian, H. H. (1997) · Nature Genetics · doi:10.1038/ng0597-37 · Cited by 451
Park, S., Kelly, R., Kahn, J. N., Robles, J., Hsu, M.-J., Register, E., Li, W., Vyas, V., Fan, H., Abruzzo, G., et al. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.8.3264-3273.2005 · Cited by 434
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
Doyon, Y., Vo, T. D., Mendel, M. C., Greenberg, S. G., Wang, J., Xia, D. F., Miller, J. C., Urnov, F. D., Gregory, P. D., & Holmes, M. C. (2011) · Nature Methods · doi:10.1038/nmeth.1539 · Cited by 402
Coupling of Gab1 to C-Met, Grb2, and Shp2 Mediates Biological Responses
Schaeper, U., Gehring, N. H., Fuchs, K. P., Sachs, M., Kempkes, B., & Birchmeier, W. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.149.7.1419 · Cited by 346
Metadata
- Authors
- Muhlrad, D., Hunter, R., & Parker, R.
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 1992-02
- Volume
- 8
- Issue
- 2
- Pages
- 79-82
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W1975593876
- Cited by
- 452
- References
- 0
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A rapid method for localized mutagenesis of yeast genes. https://app.cochat.ai/papers/10.1002/yea.320080202
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- Muhlrad, D., Hunter, R., & Parker, R. (1992). A rapid method for localized mutagenesis of yeast genes. Yeast. https://doi.org/10.1002/yea.320080202
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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