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Kinetics and Mechanisms of Hypochlorous Acid Reactions
Abstract
Hypochlorous acid (HOCl) is a strong oxidant formed in neutrophils by the myeloperoxidase-catalyzed oxidation of chloride. Using stopped-flow with spectrophotometric detection, HOCl was found to react very rapidly with glutathione and ascorbate and less rapidly with taurine. No evidence could be found for the formation of reactive free-radical intermediates in these reactions, in support of an electrophilic mechanism. In contrast, the reaction with iron(II) aquo or citrate complexes (k approximately 10(4) dm3 mol-1 s-1 in acidic solution) yielded reactive intermediates distinguishable from hydroxyl radicals. The reaction between HOCl and ferrous ions, which is analogous to but faster than the Fenton reaction, is a potential source of free radicals in activated neutrophils.
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“Hypochlorous acid (HOCl) is generated by the enzyme MPO, localized within leukocytes by the following reaction (300) (Eq. 25)”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [300]
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Metadata
- Authors
- Folkes, L. K., Candeias, L. P., & Wardman, P.
- Affiliations
- Mount Vernon Hospital (GB)
- ORCID
- Wardman, Peter
- Venue
- Archives of Biochemistry and Biophysics
- Publisher
- Elsevier BV
- Published
- 1995-10
- Volume
- 323
- Issue
- 1
- Pages
- 120-126
- ISSN
- 0003-9861 (ISSN-L)
- OpenAlex
- W1972880155
- Cited by
- 358
- References
- 0
- Citations by year
- 2026: 5 · 2025: 21 · 2024: 12 · 2023: 7 · 2022: 21 · 2021: 16 · 2020: 12 · 2019: 16 · 2018: 8 · 2017: 11 · 2016: 10 · 2015: 10 · 2014: 10 · 2013: 16 · 2012: 20
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Biochemistry and Biophysics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- hypochlorous acid, reaction kinetics, reaction mechanism, glutathione, vitamin C, taurine, iron(II) complexes, ferrous iron oxidation, Fenton reaction, hydroxyl radicals, radical intermediates, myeloperoxidase
- MeSH terms
- Ascorbic Acid, Free Radicals, Glutathione, Hypochlorous Acid, Kinetics, Taurine
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