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Cited by 75Sperm and Testicular FunctionAntioxidant Activity and Oxidative StressHeavy Metal Exposure and Toxicity

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Dose-Dependent Increase of Oxidative Damage in the Testes of Rats Subjected to Acute Iron Overload

Lucesoli, Florencia, Caligiuri, Marina, Roberti, Maria F., Perazzo, Juan C., Fraga, Cesar G.·1999·Archives of Biochemistry and Biophysics
DOI 10.1006/abbi.1999.1476
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Abstract

This study describes the in vivo response of rat testes to acute iron overload. Male Wistar rats (250-300 g) were injected ip with iron dextran at doses of 250 (Fe250), 500 (Fe500), or 1000 mg/kg body wt (Fe1000) or with saline (C). Parameters of oxidative stress and iron toxicity were measured 20 h after injection. Total iron content was 3.5-, 5.3-, and 10.4-fold higher in the Fe250, Fe500, and Fe1000 groups, respectively, compared to controls (320 +/- 22 nmol/g tissue). Histological studies showed that: (a) iron accumulated in the sperm and other testes cells, and (b) spermatogenesis was markedly lower in the Fe1000 group. The concentration of alpha-tocopherol, ubiquinol-9, and ubiquinol-10 in the testes was inversely correlated with the extent of oxidation. Testes chemiluminescence was 45% higher in the Fe1000 group compared to controls (41 cps/cm(2)). Endogenous levels of lipid oxidation, evaluated as 2-thiobarbituric acid-reactive substances, were 46, 73, and 82% higher in the groups Fe250, Fe500, and Fe1000, respectively, than in controls (33.6 +/- 1.4 nmol/g tissue). Oxidative damage to DNA evaluated by the presence of 8-oxo-2'-deoxyguanosine (oxo(8)dG), was 26, 39, and 74% higher in the Fe250, Fe500, and Fe1000 groups, respectively, than in the C group (2.3 +/- 0.1 oxo(8)dG/10(5)dG). Protein oxidation was measured as protein thiols and carbonyl content in proteins and glutamine synthase activity. Protein thiols content and glutamine synthase activity were similar in all the groups, while the protein-associated carbonyls content was 96% higher in the Fe1000 group than in the C group (2.1 +/- 0.4 nmol/mg protein). No changes in the activities of superoxide dismutase, catalase, and glutathione peroxidase were observed. The results showed that in vivo iron overload induced oxidative stress and the impairment of spermatogenesis in rat testes that were dependent on the amount of iron supplemented and its accumulation in the tissue.

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Research works printed but not linked: 41

  1. Crawford, 1995, Biochem. Mol. Med., 54, 1, 10.1006/bmme.1995.1001 · DOI
  2. Edwards, 1980, Ann. Intern. Med., 93, 519, 10.7326/0003-4819-93-4-519 · DOI
  3. Kelly, 1984, Ann. Intern. Med., 101, 629, 10.7326/0003-4819-101-5-629 · DOI
  4. Whittaker, 1997, Toxicol. Pathol., 25, 556, 10.1177/019262339702500604 · DOI
  5. Lucesoli, 1995, Arch. Biochem. Biophys., 316, 567, 10.1006/abbi.1995.1076 · DOI
  6. Lucesoli, 1999, Toxicology, 132, 179, 10.1016/S0300-483X(98)00152-8 · DOI
  7. Oteiza, 1995, J. Nutr., 125, 823
  8. Al-Bayati, 1988, Xenobiotica., 18, 1281, 10.3109/00498258809042251 · DOI
  9. Ray, 1981, J. Endocrinol. Invest., 4, 167, 10.1007/BF03350446 · DOI
  10. Fraga, 1991, Proc. Natl. Acad. Sci. USA, 88, 11003, 10.1073/pnas.88.24.11003 · DOI
  11. Fraga, 1996, Mut. Res., 351, 199, 10.1016/0027-5107(95)00251-0 · DOI
  12. Bralet, 1992, Biochem. Pharmacol., 43, 979, 10.1016/0006-2952(92)90602-F · DOI
  13. Motchnik, 1994, Methods Enzymol., 234, 269, 10.1016/0076-6879(94)34094-3 · DOI
  14. Lykkesfeldt, 1995, Anal. Biochem., 229, 329, 10.1006/abio.1995.1421 · DOI
  15. Flohé, 1984, Methods Enzymol., 105, 93, 10.1016/S0076-6879(84)05013-8 · DOI
  16. Chance, 1979, Physiol. Rev., 59, 527, 10.1152/physrev.1979.59.3.527 · DOI · Paper page
  17. Flohé, 1984, Methods Enzymol., 105, 114, 10.1016/S0076-6879(84)05015-1 · DOI
  18. Boveris, 1980, Proc. Natl. Acad. Sci. USA, 77, 347, 10.1073/pnas.77.1.347 · DOI
  19. Fraga, 1987, Free Radic. Biol. Med., 3, 119, 10.1016/S0891-5849(87)80006-0 · DOI
  20. Gupta, 1984, Proc. Natl. Acad. Sci. USA, 81, 6943, 10.1073/pnas.81.22.6943 · DOI
  21. Shigenaga, 1990, Methods Enzymol., 234, 16, 10.1016/0076-6879(94)34073-0 · DOI
  22. Levine, 1990, Methods Enzymol., 186, 464, 10.1016/0076-6879(90)86141-H · DOI
  23. Oliver, 1987, J. Biol. Chem., 262, 5488, 10.1016/S0021-9258(18)45598-6 · DOI
  24. Lou, 1987, Methods Enzymol., 143, 124, 10.1016/0076-6879(87)43022-X · DOI
  25. Murphy, 1989, Biochem. J., 260, 359, 10.1042/bj2600359 · DOI
  26. Miller, 1978, Proc. Natl. Acad. Sci. USA, 75, 1418, 10.1073/pnas.75.3.1418 · DOI
  27. Rowe, 1970, Methods Enzymol., 17, 900, 10.1016/0076-6879(71)17304-1 · DOI
  28. Bradford, 1976, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  29. Lowry, 1951, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  30. Galleano, 1994, Toxicology, 93, 125, 10.1016/0300-483X(94)90073-6 · DOI
  31. Minotti, 1987, J. Biol. Chem., 262, 1098, 10.1016/S0021-9258(19)75755-X · DOI
  32. Boveris, 1983, Arch. Biochem. Biophys., 227, 534, 10.1016/0003-9861(83)90482-4 · DOI
  33. Fraga, 1984, Acta Physiol. Pharmacol. Latinoam., 34, 143
  34. Fraga, 1987, Biochem. J., 242, 383, 10.1042/bj2420383 · DOI
  35. Berlett, 1997, J. Biol. Chem., 272, 20313, 10.1074/jbc.272.33.20313 · DOI
  36. Stadtman, 1992, Science, 257, 1220, 10.1126/science.1355616 · DOI
  37. Grattagliano, 1997, J. Appl. Toxicol., 17, 307, 10.1002/(SICI)1099-1263(199709)17:5<307::AID-JAT446>3.0.CO;2-Q · DOI
  38. McKerns, 1966, Biochem. Biophys. Acta, 121, 207, 10.1016/0304-4165(66)90377-1 · DOI
  39. Saxena, 1987, Exp. Pathol., 31, 249, 10.1016/S0232-1513(87)80010-5 · DOI
  40. Sorahan, 1997, Br. J. Cancer, 75, 134, 10.1038/bjc.1997.22 · DOI
  41. Ji, 1997, J. Natl. Cancer. Inst., 89, 238, 10.1093/jnci/89.3.238 · DOI · Paper page

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Metadata

Authors
Lucesoli, F., Caligiuri, M., Roberti, M. F., Perazzo, J. C., & Fraga, C. G.
Affiliations
  • Universidad de Buenos Aires (AR)
  • Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
Venue
Archives of Biochemistry and Biophysics
Publisher
Elsevier BV
Published
1999-12
Volume
372
Issue
1
Pages
37-43
ISSN
0003-9861 (ISSN-L)
DOI
10.1006/abbi.1999.1476
OpenAlex
W2044665824
Cited by
75
References
1
Citations by year
2025: 3 · 2024: 4 · 2023: 5 · 2022: 1 · 2021: 7 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 4 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 5
FWCI
2.71
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Archives of Biochemistry and Biophysics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Pennsylvania
  • Universidad de Buenos Aires
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords
iron dextran, testis, oxidative stress, iron toxicity, lipid peroxidation, DNA oxidation, protein carbonylation, antioxidant status, alpha-tocopherol, ubiquinol, 8-hydroxy-2'-deoxyguanosine, spermatogenesis, glutamine synthetase, superoxide dismutase, catalase
MeSH terms
8-Hydroxy-2'-Deoxyguanosine, Animals, Antioxidants, Deoxyguanosine, DNA, Iron, Luminescent Measurements, Male, Proteins, Testis, Ubiquinone, Vitamin E, Lipid Peroxidation, Rats, Wistar, Thiobarbituric Acid Reactive Substances, Oxidative Stress, Iron Overload, Rats

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CoChat. (2026). Citation check: Dose-Dependent Increase of Oxidative Damage in the Testes of Rats Subjected to Acute Iron Overload. https://app.cochat.ai/papers/10.1006/abbi.1999.1476
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Lucesoli, F., Caligiuri, M., Roberti, M. F., Perazzo, J. C., & Fraga, C. G. (1999). Dose-Dependent Increase of Oxidative Damage in the Testes of Rats Subjected to Acute Iron Overload. Archives of Biochemistry and Biophysics. https://doi.org/10.1006/abbi.1999.1476