Oxidative Stress and Heat Shock Stimulate RGS2 Expression in 1321N1 Astrocytoma Cells
Abstract
RGS2, a regulators of G-protein signaling family member, regulates G-protein signaling and is itself controlled in part by regulated expression. We tested if cell stress regulates RGS2 expression in human astrocytoma 1321N1 cells. Treatment with H2O2 increased RGS2 mRNA levels time- and concentration-dependently, with 200 microM H2O2 causing an approximately eightfold increase after 2 h. Peroxynitrite and heat shock also increased RGS2 mRNA levels. H2O2-induced RGS2 expression was negatively regulated by phosphoinositide-3-kinase and extracellular signal-regulated kinases. H2O2 also concentration-dependently increased RGS2 protein levels, and the RGS2 appeared to be predominantly in the nucleus. These results demonstrate that RGS2 expression is up-regulated by cell stress.
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GABA alpha-2 receptor is implicated in stress modulation via chloride cotransporters domains, which are activated by oxidative stress responsive kinases.
“Besides the neurotransmission regulatory function of GABA receptors, it seems that GABA alpha-2 receptor is also implicated in stress modulation via chloride cotransporters domains, which are also activated by oxidative stress responsive kinases [193].”
From Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches (2016) · cites this paper as [193]
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Balmus, I. M., Ciobica, A., Antioch, I., Dobrin, R., & Timofte, D. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/3975101 · Cited by 103 · see citing works
“Besides the neurotransmission regulatory function ofGABA receptors, it seems thatGABAalpha-2 receptor is also implicated in stress modulation via chloride cotransporters domains, which are also activated by oxidative stress responsive kinases [193].”
BackgroundEndale, M., Kim, S. D., Lee, W. M., Kim, S., Suk, K., Cho, J. Y., Park, H. J., Wagley, Y., Kim, S., Oh, J.-W., et al. (2010) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00517.2008 · Cited by 29 · see citing works
- Authors
- Zmijewski, J. W., Song, L., Harkins, L., Cobbs, C. S., & Jope, R. S.
- Affiliations
- University of Alabama at Birmingham (US)
- Corresponding author
- Jope, Richard S.
- Venue
- Archives of Biochemistry and Biophysics
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 392
- Issue
- 2
- Pages
- 192-196
- ISSN
- 0003-9861 (ISSN-L)
- OpenAlex
- W2129220792
- Cited by
- 48
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Biochemistry and Biophysics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Mental Health (MH38752)
- National Institute of Neurological Disorders and Stroke (NS37768, R01NS037768)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- RGS2, regulator of G protein signaling, oxidative stress, heat shock, hydrogen peroxide, peroxynitrite, astrocytoma, 1321N1 cells, phosphatidylinositol 3-kinase, extracellular signal-regulated kinase, gene expression regulation, nuclear localization, cellular stress response
- MeSH terms
- Astrocytoma, Cell Nucleus, Dose-Response Relationship, Drug, Hot Temperature, Humans, Hydrogen Peroxide, Immunohistochemistry, RNA, Messenger, Time Factors, Tumor Cells, Cultured, Blotting, Northern, Signal Transduction, Down-Regulation, Up-Regulation, Oxidative Stress, Phosphatidylinositol 3-Kinases, Mitogen-Activated Protein Kinase 1, RGS Proteins, Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 3
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