Cited by 95Liver physiology and pathologyCancer-related Molecular PathwaysHepatocellular Carcinoma Treatment and Prognosis

Distinct Patterns of Cyclin D1 Regulation in Models of Liver Regeneration and Human Liver

Albrecht, J.H., Hu, M.Y., Cerra, F.B.·1995·Biochemical and Biophysical Research Communications

Abstract

The expression of cyclin D1 and associated cdks was examined in models of liver regeneration and human liver specimens. In mouse liver after 70% partial hepatectomy, there was > 20-fold induction of cyclin D1 mRNA and protein, beginning prior to peak DNA synthesis. In normal rat liver, basal levels of cyclin D1 protein were significantly higher than in the mouse. After hepatectomy in the rat, cyclin D1 mRNA was induced 6- to 10-fold, while the protein levels changed 20-fold in rat hepatocytes. Immunoprecipitation of cyclin D1 demonstrated its association with cdk4 but not cdk5 in regenerating liver. In human liver biopsy specimens, cyclin D1 protein was detectable in normal liver and induced 2- to 10-fold in mitotically active liver following transplantation. These results suggest that the regulation of cyclin D1 protein in human liver may more closely parallel the mouse than the rat hepatectomy model. Furthermore, cyclin expression in primary cells in culture may differ significantly from that observed in vivo.

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Crossref · 10.1006/bbrc.1995.1548

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Authors
Albrecht, J. H., Hu, M. Y., & Cerra, F. B.
Affiliations
University of Minnesota (US)
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
1995-04
Volume
209
Issue
2
Pages
648-655
ISSN
0006-291X (ISSN-L)
OpenAlex
W2043906334
Cited by
95
References
0
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 4
FWCI
6.48
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
  • Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
Keywords
cyclin D1, liver regeneration, partial hepatectomy, cyclin-dependent kinases, CDK4, cyclin-dependent kinase 5, hepatocytes, hepatocyte growth factor, species differences, cell cycle regulation
MeSH terms
Animals, Cells, Cultured, Hepatectomy, Humans, Liver Regeneration, Proto-Oncogene Proteins, RNA, Messenger, Time Factors, Oncogene Proteins, Gene Expression, Cyclins, Protein Serine-Threonine Kinases, Cyclin-Dependent Kinases, Cyclin D1, Cyclin-Dependent Kinase 4, Mice, Rats, In Vitro Techniques

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