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Rapid Upregulation of Caspase-3 in Rat Spinal Cord after Injury: mRNA, Protein, and Cellular Localization Correlates with Apoptotic Cell Death

Citron, Bruce A., Arnold, Paul M., Sebastian, Cyril, Qin, Fan, Malladi, Sundaravalli, Ameenuddin, Syed, Landis, Mary E., Festoff, Barry W.·2000·Experimental Neurology
DOI 10.1006/exnr.2000.7523
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Abstract

Although the precise mechanisms explaining loss of, and failure to regain, function after spinal cord injury are unknown, there is increasing interest in the role of "secondary cell death." One prevalent theme in cell loss in other regions of the CNS involves apoptosis executed by the intracellular caspase proteases. A recent study demonstrated that spinal cord injury rapidly increased the activation of caspase-3. Our previous studies demonstrated peak apoptosis in three of four cellular compartments 3 days after controlled contusion in the rat. We have extended these analyses to include enzyme and substrate studies of caspase subfamilies both in rostral and in caudal adjacent segments compared to the lesion site. Although presumed activation of programmed proenzyme is considered the mechanism for enhanced caspases, our novel analyses were designed to detect upregulation of gene expression. We surveyed traumatically injured spinal cord for caspase family messages with a modified differential mRNA display approach and found that the caspase-3 (CASP3) message was present and upregulated severalfold after injury. Our results clearly demonstrate that cell death in the spinal cord occurs after posttranslational activation of caspases that follow, at least for caspase-3, initial upregulation of CASP3 mRNA levels.

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Metadata

Authors
Citron, B. A., Arnold, P. M., Sebastian, C., Qin, F., Malladi, S., Ameenuddin, S., Landis, M. E., & Festoff, B. W.
Venue
Experimental Neurology
Publisher
Elsevier BV
Published
2000-12
Volume
166
Issue
2
Pages
213-226
ISSN
0014-4886
DOI
10.1006/exnr.2000.7523
Cited by
126
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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