Cited by 86Ion channel regulation and functionCardiac electrophysiology and arrhythmiasIon Transport and Channel Regulation

Gene Structures and Expression Profiles of Three Human KCND (Kv4) Potassium Channels Mediating A-Type Currents ITO and ISA

Isbrandt, Dirk, Leicher, Thorsten, Waldschütz, Ralph, Zhu, Xinran, Luhmann, Ulrich, Michel, Uwe, Sauter, Kathrin, Pongs, Olaf·2000·Genomics

Abstract

The four known members of the KCND/Kv4 channel family encode voltage-gated potassium channels. Recent studies provide evidence that members of the Kv4 channel family are responsible for native, rapidly inactivating (A-type) currents described in heart (I(TO)) and neurons (I(SA)). In this study, we cloned the human KCND1 cDNA, localized the KCND1 gene to chromosome Xp11.23-p11.3, and determined the genomic structure and tissue-specific expression of the KCND1, KCND2, and KCND3 genes, respectively. The open reading frame of Kv4. 1 is 1941 nucleotides long, predicting a protein of 647 amino acids. The deduced protein sequence of Kv4.1 shows an overall identity of 60% with Kv4.2 and Kv4.3L and corresponds to the common structure of voltage-gated potassium channels. KCND1-specific transcripts were detectable in human brain, heart, liver, kidney, thyroid gland, and pancreas, as revealed by Northern blot and RT-PCR experiments. The comparison of the expression patterns of the known Kv4 family members shows subtype specificity with significant overlaps. The KCND gene structures exhibit an evolutionarily conserved exon pattern with a large first exon containing the intracellular N-terminus and the putative membrane-spanning regions S1 to S5, as well as part of the pore region. The KCND3 gene contains an additional exon of 57 bp, which is not present in the other two KCND genes and gives rise to the C-terminal splice KCND3L variant with an insertion of 19 amino acids.

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Crossref · 10.1006/geno.2000.6117

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Authors
Isbrandt, D., Leicher, T., Waldschütz, R., Zhu, X., Luhmann, U., Michel, U., Sauter, K., & Pongs, O.
Affiliations
  • Universität Hamburg (DE)
  • University of Göttingen (DE)
Corresponding author
Isbrandt, Dirk
Venue
Genomics
Publisher
Elsevier BV
Published
2000-03
Volume
64
Issue
2
Pages
144-154
ISSN
0888-7543 (ISSN-L)
OpenAlex
W1969536341
Cited by
86
References
47
Citations by year
2026: 3 · 2025: 2 · 2024: 4 · 2022: 5 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 5 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 3
FWCI
1.57
Type
journal article
Language
en
Open access
closed
Locations
  • Genomics (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
  • GRO (submittedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
Keywords
Kv4 potassium channels, Kv4.1, Kv4.2, A-type potassium current, Ito current, voltage-gated potassium channels, gene structure, exon structure, tissue-specific gene expression, alternative splicing, cDNA cloning
MeSH terms
Chromosome Mapping, Cloning, Molecular, Electric Conductivity, Exons, Humans, Introns, Molecular Sequence Data, RNA, Messenger, Tissue Distribution, Transcription, Genetic, Potassium Channels, Ion Channel Gating, Gene Library, Gene Expression, Polymerase Chain Reaction, Sequence Alignment, Genome, Sequence Homology, Amino Acid, DNA, Complementary, Potassium Channels, Voltage-Gated, Shal Potassium Channels

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