Cited by 74Viral Infections and Immunology ResearchCytomegalovirus and herpesvirus researchCardiac Fibrosis and Remodeling

Characterisation of Murine Cytomegalovirus Myocarditis: Cellular Infiltration of the Heart and Virus Persistence

Lenzo, Jason C, Fairweather, DeLisa, Cull, Vanessa, Shellam, Geoffrey R, James(Lawson), Cassandra M·2002·Journal of Molecular and Cellular Cardiology

Abstract

Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined murine cytomegalovirus in an animal model of myocarditis using both susceptible BALB/c and resistant C57BL/6 mice. The heart infiltrating cells of BALB/c mice were found to comprise predominantly CD8+ T cells, with other cells of the CD4+ T cell, macrophage, B cell and neutrophil phenotype. Infectious MCMV titres in the heart were low and replicative virus could not be isolated beyond the first week post-infection (p.i.). Direct viral lysis of myocytes in vitro and apoptosis of cardiac cells in vivo was observed. Furthermore, viral DNA was detected in the heart of both mouse strains throughout the development of chronic disease. Viral gB RNA was detected during the first 35 days p.i. However, viral transcript for ie1 RNA but not gB RNA was found in the heart during the late stage of disease, suggesting latent viral infection of the heart. Our findings suggest that maintenance of the chronic phase of myocarditis involving post-viral immunological responses can occur in the presence of little infectious virus replication in the heart.

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Crossref · 10.1006/jmcc.2002.2003

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  • This time course is the same as observed with the autoimmune CVB3 and murine cytomegalovirus models of myocarditis and EAM.

    “This time course is the same as is observed with the autoimmune CVB3 and murine cytomegalovirus models of myocarditis and EAM (Table S1). 35,124,170,188”

    From COVID-19, Myocarditis and Pericarditis (2023) · cites this paper as [188]

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Authors
Lenzo, J. C., Fairweather, D., Cull, V., Shellam, G. R., & James(Lawson), C. M.
Affiliations
  • Murdoch University (AU)
  • The University of Western Australia (AU)
Venue
Journal of Molecular and Cellular Cardiology
Publisher
Elsevier BV
Published
2002-06
Volume
34
Issue
6
Pages
629-640
ISSN
0022-2828 (ISSN-L)
OpenAlex
W2041149901
Cited by
74
References
51
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 5 · 2020: 4 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 7
FWCI
5.70
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Molecular and Cellular Cardiology (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (UWA) (submittedVersion)
  • Murdoch University Research Portal (submittedVersion)
Funders
Chinese Academy of Agricultural Sciences
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
Keywords
murine cytomegalovirus, myocarditis, viral persistence, viral latency, cardiac inflammation, CD8+ T cells, apoptosis, strain susceptibility, BALB/c mice, C57BL/6 mice, chronic viral infection
MeSH terms
Acute Disease, Animals, Antigens, Viral, Cells, Cultured, Chronic Disease, Cytomegalovirus Infections, Cytomegalovirus, DNA, Viral, Female, Heart, Mice, Inbred BALB C, Mice, Inbred C57BL, Myocarditis, Myocardium, Phenotype, RNA, Viral, Viral Envelope Proteins, Viral Proteins, Virus Replication, Polymerase Chain Reaction, Immediate-Early Proteins, Mice

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