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Primers for a PCR-Based Approach to Mitochondrial Genome Sequencing in Birds and Other Vertebrates
Open accessAbstract
A PCR-based approach to sequencing complete mitochondrial genomes is described along with a set of 86 primers designed primarily for avian mitochondrial DNA (mtDNA). This PCR-based approach allows an accurate determination of complete mtDNA sequences that is faster than sequencing cloned mtDNA. The primers are spaced at about 500-base intervals along both DNA strands. Many of the primers incorporate degenerate positions to accommodate variation in mtDNA sequence among avian taxa and to reduce the potential for preferential amplification of nuclear pseudogenes. Comparison with published vertebrate mtDNA sequences suggests that many of the primers will have broad taxonomic utility. In addition, these primers should make available a wider variety of mitochondrial genes for studies based on smaller data sets.
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Cited by
Miya, M., Kawaguchi, A., & Nishida, M. (2001) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a003741 · Cited by 419
deWaard, J. R., Ivanova, N. V., Hajibabaei, M., & Hebert, P. D. N. (2008) · Methods in Molecular Biology · doi:10.1007/978-1-59745-548-0_15 · Cited by 389
Molaei, G., Andreadis, T. G., Armstrong, P. M., Anderson, J. F., & Vossbrinck, C. R. (2006) · Emerging Infectious Diseases · doi:10.3201/eid1203.051004 · Cited by 356
Lerner, H. R. L., & Mindell, D. P. (2005) · Molecular Phylogenetics and Evolution · doi:10.1016/j.ympev.2005.04.010 · Cited by 266
Self-citationInterordinal Relationships of Birds and Other Reptiles Based on Whole Mitochondrial Genomes
Mindell, D. P., Sorenson, M. D., Dimcheff, D. E., Hasegawa, M., Ast, J. C., & Yuri, T. (1999) · Systematic Biology · doi:10.1080/106351599260490 · Cited by 255
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Metadata
- Authors
- Sorenson, M. D., Ast, J. C., Dimcheff, D. E., Yuri, T., & Mindell, D. P.
- Venue
- Molecular Phylogenetics and Evolution
- Publisher
- Elsevier BV
- Published
- 1999-07
- Volume
- 12
- Issue
- 2
- Pages
- 105-114
- ISSN
- 1055-7903 (ISSN-L)
- OpenAlex
- W2052536191
- Cited by
- 790
- References
- 0
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sorenson, M. D., Ast, J. C., Dimcheff, D. E., Yuri, T., & Mindell, D. P. (1999). Primers for a PCR-Based Approach to Mitochondrial Genome Sequencing in Birds and Other Vertebrates. Molecular Phylogenetics and Evolution. https://doi.org/10.1006/mpev.1998.0602
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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