The DOI matches its Crossref record.
Cotransfection of HPV-18 and v-fos DNA Induces Tumorigenicity of Primary Human Keratinocytes
Abstract
Human keratinocytes were cotransfected with the FBJ/R v-fos oncogene and the HPV-18 genome and selected on the basis of their resistance to inducers of terminal differentiation. Unlike keratinocytes immortalized by HPV-18 DNA alone, the HPV-18/v-fos transformants exhibited prominent intracellular vacuolization and formed progressively growing, squamous cell tumors when injected subcutaneously into nude mice. Cytogenetic analysis of two clonally selected transformed cell lines (18/fos clone 1 and 18/fos clone 2) revealed minimal alterations in karyotype, although a consistent rearrangement of chromosome 10, iso(10q), was observed. Further analysis of 18/fos clone 1 confirmed the expression of the fos gene as well as the HPV-18 E7 protein. Alterations in fos gene expression, which appear to be an important facet of epidermal differentiation in vivo, could potentially contribute to the malignant progression of HPV immortalized cells.
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In the citing paper
“Additional oncogenic events are necessary for malignant progression to occur, such as expression of oncogenes like ras or fos, or accumulation of oncogenic mutations over prolonged passaging in culture [307,308,309,310]”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [310]
In the citing paper
“Despite the fact that expression of high-risk oncogenes in primary human keratinocytes can cause cellular immortalization, with the cells exhibiting many characteristics of premalignant lesions, these cells do not form tumours when injected into nude mice.”
From The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis (2021) · cites this paper as [310]
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Cited by
Basukala, O., & Banks, L. (2021) · Viruses · doi:10.3390/v13101892 · Cited by 137
Metadata
- Authors
- Pei, X. F., Meck, J. M., Greenhalgh, D., & Schlegel, R.
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 1993-10
- Volume
- 196
- Issue
- 2
- Pages
- 855-860
- ISSN
- 0042-6822
- Cited by
- 39
- References
- 0
- Language
- en
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- CoChat. (2026). Citation check: Cotransfection of HPV-18 and v-fos DNA Induces Tumorigenicity of Primary Human Keratinocytes. https://app.cochat.ai/papers/10.1006/viro.1993.1546
- The paper
- Pei, X. F., Meck, J. M., Greenhalgh, D., & Schlegel, R. (1993). Cotransfection of HPV-18 and v-fos DNA Induces Tumorigenicity of Primary Human Keratinocytes. Virology. https://doi.org/10.1006/viro.1993.1546
