The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Construction of Infectious cDNA Clones for Dengue 2 Virus: Strain 16681 and Its Attenuated Vaccine Derivative, Strain PDK-53
Open accessAbstract
We identified nine nucleotide differences between the genomes of dengue-2 (DEN-2) 16681 virus and its vaccine derivative, strain PDK-53. These included a C-to-T (16681-to-PDK-53) mutation at nucleotide position 57 of the 5'-untranslated region, three silent mutations, and substitutions prM-29 Asp to Val, NS1-53 Gly to Asp, NS2A-181 Leu to Phe, NS3-250 Glu to Val, and NS4A-75 Gly to Ala. Unpassaged PDK-53 vaccine contained two genetic variants as a result of partial mutation at NS3-250. We constructed infectious cDNA clones for 16681 virus and each of the two PDK-53 variants. DEN-2 16681 clone-derived viruses were identical to the 16681 virus in plaque size and replication in LLC-MK2 cells, replication in C6/36 cells, E and prM epitopes, and neurovirulence for suckling mice. PDK-53 virus and both clone-derived PDK-53 variants were attenuated in mice. However, the variant containing NS3-250-Glu was less temperature sensitive and replicated better in C6/36 cells than did PDK-53 virus. The variant containing NS3-250-Val had smaller, more diffuse plaques, decreased replication, and increased temperature sensitivity in LLC-MK2 cells relative to PDK-53 virus. Both PDK-53 virus and the NS3-250-Val variant replicated poorly in C6/36 cells relative to 16681 virus. Unpassaged PDK-53 vaccine virus and the virus passaged once in LLC-MK2 cells had genomes of identical sequence, including the mixed NS3-250-Glu/Val locus. Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice.
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Cites
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Cited by
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Dengue Virus Capsid Protein Usurps Lipid Droplets for Viral Particle Formation
Samsa, M. M., Mondotte, J. A., Iglesias, N. G., Assunção-Miranda, I., Barbosa-Lima, G., Da Poian, A. T., Bozza, P. T., & Gamarnik, A. V. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000632 · Cited by 586
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Dengue virus activates cGAS through the release of mitochondrial DNA
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Metadata
- Authors
- Kinney, R. M., Butrapet, S., Chang, G.-J. J., Tsuchiya, K. R., Roehrig, J. T., Bhamarapravati, N., & Gubler, D. J.
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 1997-04
- Volume
- 230
- Issue
- 2
- Pages
- 300-308
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2083053974
- Cited by
- 320
- References
- 22
- FWCI
- 11.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kinney, R. M., Butrapet, S., Chang, G.-J. J., Tsuchiya, K. R., Roehrig, J. T., Bhamarapravati, N., & Gubler, D. J. (1997). Construction of Infectious cDNA Clones for Dengue 2 Virus: Strain 16681 and Its Attenuated Vaccine Derivative, Strain PDK-53. Virology. https://doi.org/10.1006/viro.1997.8500
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