Vesicular Release of Ebola Virus Matrix Protein VP40
Open accessAbstract
We have analysed the expression and cellular localisation of the matrix protein VP40 from Ebola virus. Full-length VP40 and an N-terminal truncated construct missing the first 31 residues [VP40(31-326)] both locate to the plasma membrane of 293T cells when expressed transiently, while a C-terminal truncation of residues 213 to 326 [VP40(31-212)] shows only expression in the cytoplasm, when analysed by indirect immunofluorescence and plasma membrane preparations. In addition, we find that full-length VP40 [VP40(1-326)] and VP40(31-326) are both released into the cell culture supernatant and float up in sucrose gradients. The efficiency of their release, however, is dependent on the presence of the N-terminal 31 residues. VP40 that is released into the supernatant is resistant to trypsin digestion, a finding that is consistent with the formation of viruslike particles detected by electron microscopy. Together, these results provide strong evidence that Ebola virus VP40 is sufficient for virus assembly and budding from the plasma membrane.
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These findings corroborated previous observations that VP40 is sufficient to generate Ebola virus-like particles that form by budding off from host plasma membranes.
“These findings corroborated previous observations that VP40 is sufficient to generate Ebola virus-like particles that form by budding off from host plasma membranes [79,80].”
From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [80]
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- Authors
- Timmins, J., Scianimanico, S., Schoehn, G., & Weissenhorn, W.
- Affiliations
- European Molecular Biology Laboratory (FR)
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 283
- Issue
- 1
- Pages
- 1-6
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2050499016
- Cited by
- 190
- References
- 29
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 6 · 2019: 8 · 2018: 9 · 2017: 9 · 2016: 8 · 2015: 10 · 2014: 10 · 2013: 6 · 2012: 9
- FWCI
- 10.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Immunology and Microbiology › Virology › Poxvirus research and outbreaks
- Keywords
- Ebola virus, VP40, matrix protein, vesicle release, plasma membrane localization, viral assembly, budding, virus-like particles, N-terminal truncation, trypsin resistance, cytoplasmic retention
- MeSH terms
- Cell Line, Cell Membrane, Centrifugation, Density Gradient, Culture Media, Fluorescent Antibody Technique, Humans, Microscopy, Electron, Nucleoproteins, Transfection, Viral Core Proteins, Blotting, Western, Ebolavirus
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