Proximity ligation: A specific and versatile tool for the proteomic era
Abstract
Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.
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In situ PLA has a resolution of 25-30 nm, so the authors cannot exclude that they may detect D2R-OXTR heteroreceptor complexes and/or co-location within the same cell membrane nanodomains.
“As in situ PLA typically provides a resolution in the range of 25–30 nm when secondary IgG antibodies are used (Soderberg et al., 2006; Soderberg et al., 2007), we cannot exclude the possibility that in our current experiments, we may detect D2R–OXTR heteroreceptor complexes and/or the co-location of D2R and OXTR within the same cell membrane nanodomains (hereafter referred to as heterocomplexes).”
From Evidence for the existence of facilitatory interactions between the dopamine D2 receptor and the oxytocin receptor in the amygdala of the rat relevance for anxiolytic actions (2023) · cites this paper as Soderberg et al., 2007
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- Authors
- Söderberg, O., Leuchowius, K.-J., Kamali-Moghaddam, M., Jarvius, M., Gustafsdottir, S., Schallmeiner, E., Gullberg, M., Jarvius, J., & Landegren, U.
- Affiliations
- Uppsala University (SE)
- Venue
- Genetic Engineering
- Publisher
- Springer US
- Published
- 2007
- Volume
- 28
- Pages
- 85-93
- OpenAlex
- W1587346581
- Cited by
- 59
- References
- 2
- Citations by year
- 2023: 2 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 6 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 11 · 2013: 5 · 2012: 5
- FWCI
- 3.66
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Springer eBooks (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- proximity ligation, protein detection, proteomics, protein-protein interaction, post-translational modification, alternative splicing, single-cell analysis, biological fluids, detection method comparison
- MeSH terms
- Animals, Genetic Engineering, Humans, Proteomics
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