Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung
Open accessAbstract
Pulmonary macrophages are heterogeneous. Distinct populations of resident tissue macrophages exist in the lung airspace and tissue compartments during homeostasis. During inflammation, these are joined by monocyte-derived recruited macrophages. Flow cytometry can be used to identify and purify lung macrophage subsets. Here, we describe methods for identifying and isolating macrophages from bronchoalveolar lavage and digested lung tissues from mouse and human. We also describe basic staining for flow cytometry analysis of different macrophage subsets.
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TREM2 promotes lung fibrosis via controlling alveolar macrophage survival and pro-fibrotic activity
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“Mo-AMs and TR-AMs can be readily separated by fl ow cytometry based on a set of typical cell surface markers 8 – 12 .”
- Authors
- King, E. M., Hume, P. S., Janssen, W. J., & McCubbrey, A. L.
- Affiliations
- National Jewish Health (US)
- Corresponding author
- McCubbrey, Alexandra L.
- Venue
- Methods in Molecular Biology
- Publisher
- Springer US
- Published
- 2022
- Volume
- 2506
- Pages
- 257-267
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC10978154
- OpenAlex
- W4285170676
- Cited by
- 7
- References
- 13
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 1
- FWCI
- 3.26
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R35HL140039, R01HL130938)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Keywords
- lung macrophages, macrophage subsets, bronchoalveolar lavage, tissue digestion, monocyte-derived macrophages, lung inflammation, mouse lung, human lung
- MeSH terms
- Animals, Bronchoalveolar Lavage Fluid, Flow Cytometry, Humans, Lung, Macrophages, Macrophages, Alveolar, Mice
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