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Cited by 261

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Techniques in HIV Research

·1990·Palgrave Macmillan UK eBooks
DOI 10.1007/978-1-349-11888-5
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Abstract

Part 1 Antibody detection in patient's serum: ELISA assay western blot analysis. Part 2 Virus detection in pathological specimen: p24 assay polymerase chain reaction (PCR) in situ hybridization southern blot analysis. Part 3 Virus isolation and production: isolation from periperal blood isolation from tissues virus production in stable cell lines. Part 4 Virus titration: on MT4 cells on HeLa cells on C81-66 on SUP T1. Part 5 Virus detection in cell lines: reverse transcriptase assay p24 assay immunofluorescence (IF) cythopathic effect (CPE) radioimmunoprecipitation (RIP). Part 6 Virus purification and storage. Part 7 Proviral DNA cloning: recombinant DNA libraries polymerase chain reaction (PCR). Part 8 Tranfection of biologically active molecular clones: DEAE dextran electroporation CaPO4 transfection lipofection selection of stably transfected cell lines. Part 9 Host immune response: neutralizing antibodies ADCC/CMC cytotoxity - CTL proliferative response. Part 10 Cytokine assays. Part 11 Antiviral drugs assays. Appendices: source of reagents biosafey issues.

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Metadata

Venue
Palgrave Macmillan UK eBooks
Publisher
Palgrave Macmillan UK
Published
1990
DOI
10.1007/978-1-349-11888-5
OpenAlex
W1496528528
Cited by
261
References
0
FWCI
5.82
Type
book
Language
en
Open access
closed

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CoChat. (2026). Citation check: Techniques in HIV Research. https://app.cochat.ai/papers/10.1007/978-1-349-11888-5
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Techniques in HIV Research. (1990). Palgrave Macmillan UK eBooks. https://doi.org/10.1007/978-1-349-11888-5

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