One-stage factor VIII assays
Abstract
Coagulation factor assays using one-stage methodology are widely used to measure factor levels for the purpose of detecting a deficiency, or to monitor replacement therapy. In this chapter, we focus on a method to measure Factor VIII coagulant activity (FVIII:C) by the one-stage assay (FVIII:C-1), with extra information provided to also allow this method to apply to Factor IX (FIX), Factor XI (FXI), and Factor XII (FXII). From the perspective of laboratory testing, these factors are components of the "intrinsic" coagulation pathway and are all measured in test systems based on the correction of the Activated Partial Thromboplastin Time (APTT). Factor activity is assessed by measuring the ability of an unknown sample to correct the prolonged APTT of factor-deficient plasma (deficient in the factor of interest), relative to the effect of a known calibrator. These assays are used for the diagnosis of the many causes of reduced factor levels, including those causing a prolonged APTT. It is important for laboratory staff to understand the impact of method variations, limitations, and result interpretation and these aspects are also discussed.
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A one-stage assay using multiple dilutions can check the presence of inhibitors while simultaneously measuring factor activity.
“The assay can check the presence of inhibitors while simultaneously measuring factor activity [16].”
From Performance characteristics of 5 numerical indexes in mixing test interpretation under coexistence of lupus anticoagulant and coagulation factor deficiency (2023) · cites this paper as [16]
Cites
Development of the human coagulation system in the full-term infant
Andrew, M., Paes, B. A., MILNER, R. A., et al. (1987) · Blood · doi:10.1182/blood.v70.1.165.165 · Cited by 1,187 · see citing works
White, G. C., Rosendaal, F. R., Aledort, L. M., Lusher, J. M., Rothschild, C., & Ingerslev, J. (2001) · PubMed · Cited by 798 · see citing works
Rosendaal, F. R., Aledort, L. M., Lusher, J. M., Rothschild, C., Ingerslev, J., & White, G. (2001) · Thrombosis and Haemostasis · doi:10.1055/s-0037-1615621 · Cited by 787 · see citing works
Barnes, C. D., Ignjatović, V., Furmedge, J., Newall, F. H., Chan, A. K. C., Rosa, L. D., Hamilton, S., Ragg, P., Robinson, S., Auldist, A., et al. (2006) · Thrombosis and Haemostasis · doi:10.1160/th05-01-0047 · Cited by 517 · see citing works
Guidelines on the laboratory aspects of assays used in haemostasis and thrombosis
Mackie, I. J., COOPER, P. C., Lawrie, A. S., Kitchen, S., Gray, E., & Laffan, M. (2012) · International Journal of Laboratory Hematology · doi:10.1111/ijlh.12004 · Cited by 161 · see citing works
A critical appraisal of one‐stage and chromogenic assays of factor VIII activity
Peyvandi, F., Oldenburg, J., & Friedman, K. D. (2015) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.13215 · Cited by 155 · see citing works
Influence of phospholipids on the assessment of factor VIII activity
Lee, C. A., Kessler, C. M., Varon, D., Martinowitz, U. P., Heim, M., Mikaelsson, M., Oswaldsson, U., & Sandberg, H. (1998) · Haemophilia · doi:10.1046/j.1365-2516.1998.440646.x · Cited by 119 · see citing works
Bonar, R. A., Favaloro, E. J., Mohammed, S., et al. (2015) · Pathology · doi:10.1016/j.pathol.2015.11.025 · Cited by 110 · see citing works
Barrowcliffe, T. W., Raut, S., Sands, D., & Hubbard, A. R. (2002) · Seminars in Thrombosis and Hemostasis · doi:10.1055/s-2002-32658 · Cited by 99 · see citing works
Favaloro, E. J., Soltani, S., & McDonald, J. C. (2004) · American Journal of Clinical Pathology · doi:10.1309/e4947dg48tvy19c2 · Cited by 79 · see citing works
Inhibitor development and mortality in non‐severe hemophilia A
Eckhardt, C. L., Loomans, J. I., van Velzen, A. S., et al. (2015) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12990 · Cited by 77 · see citing works
Current laboratory practices in the diagnosis and management of haemophilia: a global assessment
Kitchen, S., Signer-Romero, K., & Key, N. S. (2015) · Haemophilia · doi:10.1111/hae.12639 · Cited by 69 · see citing works
Nitu‐Whalley, Lee, Griffioen, et al. (2000) · British Journal of Haematology · doi:10.1046/j.1365-2141.2000.01830.x · Cited by 69 · see citing works
Favaloro, E. J., Soltani, S., McDonald, J. C., et al. (2005) · Blood Coagulation & Fibrinolysis · doi:10.1097/01.mbc.0000187250.32630.56 · Cited by 60 · see citing works
Nijmegen-Bethesda Assay to Measure Factor VIII Inhibitors
Duncan, E. M., Collecutt, M. F., & Street, A. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-339-8_24 · Cited by 54 · see citing works
Self-citationEvaluation of Pre-analytical Variables in a Commercial Thrombin Generation Assay
Rodgers, S., Wong, A., Gopal, R. D., et al. (2014) · Thrombosis Research · doi:10.1016/j.thromres.2014.04.010 · Cited by 43 · see citing works
Self-citationDeterminants of factor VIII plasma levels in carriers of haemophilia A and in control women
Ay, C., Thom, K., ABU‐HAMDEH, F., et al. (2009) · Haemophilia · doi:10.1111/j.1365-2516.2009.02108.x · Cited by 42 · see citing works
Lyall, H., HILL, M., Westby, J. C., et al. (2007) · Haemophilia · doi:10.1111/j.1365-2516.2007.01557.x · Cited by 39 · see citing works
Rodgers, S., McRae, S. J., & Duncan, E. M. (2013) · Seminars in Thrombosis and Hemostasis · doi:10.1055/s-0033-1334863 · Cited by 38 · see citing works
Self-citationDiagnosis of factor VIII deficiency
Verbruggen, B., Meijer, P., Nováková, I. R. O., & van Heerde, W. L. (2008) · Haemophilia · doi:10.1111/j.1365-2516.2008.01715.x · Cited by 38 · see citing works
Preston, F. E., Kitchen, S., Jennings, I., et al. (2004) · Journal of Thrombosis and Haemostasis · doi:10.1046/j.1538-7836.2003.00447.x · Cited by 34 · see citing works
Problems and Solutions in Laboratory Testing for Hemophilia
Meijer, P., Jennings, I., Sioufi, J., et al. (2013) · Seminars in Thrombosis and Hemostasis · doi:10.1055/s-0033-1360721 · Cited by 34 · see citing works
Manco‐Johnson, M. J., Nuss, R., & Jacobson, L. J. (2000) · Journal of Laboratory and Clinical Medicine · doi:10.1067/mlc.2000.107299 · Cited by 30 · see citing works
New developments in laboratory diagnosis and monitoring
KITCHEN, S., Hayward, C. P. M., Négrier, C., et al. (2010) · Haemophilia · doi:10.1111/j.1365-2516.2010.02295.x · Cited by 24 · see citing works
External Quality Assessment of Factor VIII Inhibitor Assays
Favaloro, E. J., MARSDEN, K. A., & Bonar, R. (2013) · Seminars in Thrombosis and Hemostasis · doi:10.1055/s-0033-1334464 · Cited by 20 · see citing works
Hubbard, A. R., Hamill, M. L., BEEHARRY, M., Bevan, S. A., & Heath, A. (2011) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2011.04471.x · Cited by 13 · see citing works
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Matsuda, M., Hoshiyama, Y., Ogawa, K., Emmi, M., Terai, S., & Moriyama, M. (2023) · Research and Practice in Thrombosis and Haemostasis · doi:10.1016/j.rpth.2023.100065 · Cited by 4 · see citing works
“One-stage FVIII assays were performed on samples containing antiPS/PT and showing correction in mixing tests, using multiple sample dilutions doubling from 1/5 to 1/80, to check parallelism [16,17].”
BackgroundMethodResultInfluential
- Authors
- Duncan, E., & Rodgers, S.
- Affiliations
- South Australia Pathology (AU)
- Corresponding author
- Duncan, Elizabeth
- Venue
- Methods in molecular biology
- Publisher
- Springer New York
- Published
- 2017
- Volume
- 1646
- Pages
- 247-263
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2743474746
- Cited by
- 35
- References
- 26
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 2 · 2017: 2
- FWCI
- 12.90
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Hemophilia Treatment and Research
- Health Sciences › Medicine › Genetics › Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- factor VIII coagulant activity, intrinsic coagulation pathway, factor IX, factor XI, factor XII, interpretation of results
- MeSH terms
- Blood Coagulation, Factor IX, Factor VIII, Factor XII, Hemophilia A, Humans, Partial Thromboplastin Time, Quality Control
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