The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Experimental Validation of MicroRNA Targets: Luciferase Reporter Assay
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs of 22-25 nucleotides that control gene expression at the posttranscriptional level through the degradation of mRNAs or translational repression. In the last 15 years, the study of these small molecules helped elucidate their role in the regulation of many cellular processes and the onset and development of several diseases. Therefore, many computational tools based on algorithms for target prediction have been developed to identify potential miRNA-target interactions. The improvement of experimental approaches to more easily and quickly confirm in silico predictions has become essential for the study of these small RNAs and their molecular functions. In this chapter, we summarized the principal steps of one of the most used techniques for the validation of microRNA targets, the Luciferase assay, thus explaining the underlying principles and the procedures to apply it best.
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Bacterial transformation: distribution, shared mechanisms and divergent control
Johnston, C., Martin, B., Fichant, G. A., Polard, P., & Claverys, J. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3199 · Cited by 802
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
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Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment
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An equation for calculating the volumetric ratios required in a ligation reaction
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Metadata
- Authors
- Tomasello, L., Cluts, L., & Croce, C. M.
- Affiliations
- The Ohio State University (US)
- Corresponding author
- Tomasello, Luisa
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2019
- Volume
- 1970
- Pages
- 315-330
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2940180249
- Semantic Scholar
- 8978780b8b6a278ba4cd9c70e7764f6e079af961
- Cited by
- 18
- References
- 7
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 5 · 2022: 3 · 2021: 3
- FWCI
- 1.75
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- microRNA, miRNA target validation, luciferase reporter assay, post-transcriptional regulation, miRNA-target interactions, protein synthesis inhibition, mRNA degradation
- MeSH terms
- Gene Expression Regulation, Humans, Luciferases, RNA, Messenger, Genes, Reporter, Computational Biology, MicroRNAs, Validation Studies as Topic
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