The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
Open accessAbstract
Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate pro tein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpression and copurification of interacting proteins, or in vivo removal of MBP by TVMV protease to improve screening and purification. All target genes and vectors are processed by the same protocols, which we describe here.
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An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
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Seiler, C. Y., Park, J. G., Sharma, A., Hunter, P., Surapaneni, P., Sedillo, C., Field, J., Algar, R., Price, A., Steel, J., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1060 · Cited by 224
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Metadata
- Authors
- Eschenfeldt, W. H., Lucy, S., Millard, C. S., Joachimiak, A., & Mark, I. D.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2009
- Volume
- 498
- Pages
- 105-115
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W1508587432
- Cited by
- 268
- References
- 11
- FWCI
- 5.26
- Type
- book chapter
- Language
- en
- Open access
- green
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- Eschenfeldt, W. H., Lucy, S., Millard, C. S., Joachimiak, A., & Mark, I. D. (2009). A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins. Methods in Molecular Biology. https://doi.org/10.1007/978-1-59745-196-3_7
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