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Cited by 318Reproductive Biology and FertilityMolecular Biology Techniques and ApplicationsPlant tissue culture and regeneration

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Cryopreservation and Freeze-Drying Protocols

John Day, Glyn Stacey·2007·Methods in Molecular Biology
DOI 10.1007/978-1-59745-362-2
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Abstract

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

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Metadata

Authors
Day, J., & Stacey, G.
Affiliations
National Institute for Biological Standards and Control (GB)
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2007
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-59745-362-2
OpenAlex
W1506233354
Cited by
318
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 11 · 2023: 15 · 2022: 15 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 21 · 2017: 25 · 2016: 14 · 2015: 17 · 2014: 16 · 2013: 23 · 2012: 27
FWCI
13.41
Type
book
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Methods in molecular biology (publishedVersion)
  • DIAL (Catholic University of Leuven) (submittedVersion)
Funders
  • NHS Blood and Transplant
  • University of Bedfordshire
  • Cardiff University
  • Directorate for Biological Sciences
  • Biotechnology and Biological Sciences Research Council
  • Universitätsklinikum Hamburg-Eppendorf
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
Keywords
cryopreservation, freeze-drying, ex situ conservation, biological resource centers, protein preservation, long-term preservation

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CoChat. (2026). Citation check: Cryopreservation and Freeze-Drying Protocols. https://app.cochat.ai/papers/10.1007/978-1-59745-362-2
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Day, J., & Stacey, G. (2007). Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology. https://doi.org/10.1007/978-1-59745-362-2