Cited by 318Reproductive Biology and FertilityMolecular Biology Techniques and ApplicationsPlant tissue culture and regeneration

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Cryopreservation and Freeze-Drying Protocols

John Day, Glyn Stacey·2007·Methods in Molecular Biology

DOI 10.1007/978-1-59745-362-2

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Abstract

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995. This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

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Authors: Day, J., & Stacey, G.
Affiliations: National Institute for Biological Standards and Control (GB)
Venue: Methods in Molecular Biology
Publisher: Humana Press
Published: 2007
ISSN: 1064-3745 (ISSN-L)
1940-6029
DOI: 10.1007/978-1-59745-362-2
OpenAlex: W1506233354
Cited by: 318
References: 0
Citations by year: 2026: 2 · 2025: 5 · 2024: 11 · 2023: 15 · 2022: 15 · 2021: 21 · 2020: 16 · 2019: 19 · 2018: 21 · 2017: 25 · 2016: 14 · 2015: 17 · 2014: 16 · 2013: 23 · 2012: 27
FWCI: 13.41
Type: book
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Methods in molecular biology (publishedVersion)
DIAL (Catholic University of Leuven) (submittedVersion)
Funders: NHS Blood and Transplant
University of Bedfordshire
Cardiff University
Directorate for Biological Sciences
Biotechnology and Biological Sciences Research Council
Universitätsklinikum Hamburg-Eppendorf
Topics: Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
Keywords: cryopreservation, freeze-drying, ex situ conservation, biological resource centers, protein preservation, long-term preservation

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Cryopreservation and Freeze-Drying Protocols

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