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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues
Abstract
In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
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“Ultra Performance Liquid Chromatography (UPLC) coupled to electrospray ionization quadrupole time-of-flight mass spectrometry (QTOFMS) was used with the established protocol87.”
From Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses (2016) · cites this paper as [87]
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Metadata
- Authors
- Nygren, H., Seppänen-Laakso, T., Castillo, S., Hyötyläinen, T., & Orešič, M.
- Affiliations
- VTT Technical Research Centre of Finland (FI)
- Corresponding author
- Orešič, Matej
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2010-12-17
- Volume
- 708
- Pages
- 247-257
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W207791811
- Cited by
- 154
- References
- 18
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 9 · 2021: 11 · 2020: 10 · 2019: 8 · 2018: 7 · 2017: 10 · 2016: 10 · 2015: 9 · 2014: 19 · 2013: 16 · 2012: 12
- FWCI
- 14.31
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- lipidomics, serum lipids, tissue lipids, biological fluids, Folch extraction, UPLC-MS, accurate mass, LTQ-Orbitrap, sample preparation
- MeSH terms
- Body Fluids, Chromatography, High Pressure Liquid, Humans, Lipids, Mass Spectrometry, Clinical Chemistry Tests, Analytic Sample Preparation Methods
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