Models of Neurodegenerative Disease – Alzheimer’s Anatomical and Amyloid Plaque Imaging
Open accessAbstract
Alzheimer's disease (AD) is an important social and economic issue for our societies. The development of therapeutics against this severe dementia requires assessing the effects of new drugs in animal models thanks to dedicated biomarkers. According to the amyloid cascade hypothesis, β-amyloid deposits are at the origin of most of the lesions associated with AD. These extracellular deposits are therefore one of the main targets in therapeutical strategies. Aβ peptides can be revealed histologically with specific dyes or antibodies, or by magnetic resonance microscopy (μMRI) that uses their association with iron as a source of signal. The microscopic size of the lesions necessitates the development of specific imaging protocols. Most protocols use T (2)-weighted sequences that reveal the aggregates as hypointense spots. This chapter describes histological methods that reveal amyloid plaques with specific stains and MR-imaging protocols for in vivo and ex vivo MR imaging of AD mice.
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Senile plaques have been observed with MRI in postmortem human tissues and Alzheimer's disease transgenic mice.
“Although senile plaques have been observed with MRI in both postmortem human tissues and AD transgenic mice, 7”
From Amyloid Plaque Imaging with a Targeted MRI Contrast Agent in a Transgenic Mouse Model of Alzheimer’s Disease (2022) · cites this paper as [7]
The Introduction says the chapter describes MRI methods for detecting amyloid plaques in vivo and ex vivo in transgenic AD mice. It does not report MRI observation of plaques in postmortem human tissue; its discussion of human plaque imaging instead concerns PET. That human-tissue part of the claim is unsupported by our copy. (LLM, conf=0.86)
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- Authors
- Petiet, A., Delatour, B., & Dhenain, M.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- ORCID
- Petiet, Alexandra
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2011
- Volume
- 771
- Pages
- 293-308
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC3866378
- OpenAlex
- W304122992
- Cited by
- 14
- References
- 22
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2018: 1 · 2017: 2 · 2014: 2 · 2012: 2
- FWCI
- 6.23
- Type
- book chapter
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Aging (R01AG020197)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- Alzheimer's disease, amyloid plaques, amyloid-beta, amyloid hypothesis, animal models of Alzheimer's disease, magnetic resonance microscopy, T2-weighted imaging, ex vivo imaging, in vivo imaging, mouse model
- MeSH terms
- Alzheimer Disease, Animals, Brain, Magnetic Resonance Imaging, Neurodegenerative Diseases, Mice, Plaque, Amyloid
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