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Cited by 132Virus-based gene therapy researchRNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in Insects

Adeno-Associated Virus Biology

Weitzman, Matthew D., Linden, R. Michael·2012·Methods in Molecular Biology
DOI 10.1007/978-1-61779-370-7_1
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Abstract

Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined the sequence of the genome, and defined the genetic elements of the virus. The refinement of methods and protocols for the production and application of rAAV vectors has come from years of studies that explored the basic biology of this virus and its interaction with host cells. Interest in improving vector performance has in turn driven studies that have provided tremendous insights into the basic biology of the AAV lifecycle. In this chapter, we review the background on AAV biology and its exploitation for vectors and gene delivery.

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  • For productive replication, AAVs depend on coinfection with helper viruses such as adenoviruses or herpesviruses.

    “For productive replication, the AAVs depend on the coinfection with helper viruses, such as, adenoviruses or herpesviruses [2].”

    From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [2]

  • Herpes virus directly interacts with AAV replication with its viral helicase-primase complex.

    “Furthermore, herpes virus directly interacts with AAV replication with its viral helicase– primase complex [2].”

    From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [2]

  • Helper viruses provide transcription factors for AAV promoters, replication factors, and factors that modify the host cell environment for efficient AAV transcription, translation and genome replication.

    “The functions that these helper viruses provide include transcription factors for the AAV promoters, replication factors and factors that modify the host cell environment for efficient AAV transcription, translation and genome replication [2].”

    From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [2]

  • Single-stranded binding proteins E2A (adenovirus) or ICP8 (herpes virus) provided by helper viruses are involved in displacement of the ssDNA genome.

    “In that process, the single-stranded binding proteins, E2A (adenovirus) or ICP8 (herpes virus) provided by the helper viruses are involved [2, 51].”

    From Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses (2017) · cites this paper as [2]

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Authors
Weitzman, M. D., & Linden, R. M.
Affiliations
  • Salk Institute for Biological Studies (US)
  • King's College London (GB)
Corresponding author
Linden, R. Michael
ORCID
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2011-09-27
Volume
807
Pages
1-23
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-61779-370-7_1
OpenAlex
W191504613
Cited by
132
References
14
Citations by year
2026: 2 · 2025: 15 · 2024: 7 · 2023: 8 · 2022: 10 · 2021: 18 · 2020: 16 · 2019: 12 · 2018: 7 · 2017: 11 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 5 · 2012: 2
FWCI
9.83
Type
book chapter
Language
en
Open access
closed
Licence
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (King's College London) (submittedVersion)
Funders
  • National Institutes of Health (5r01ca097093-13)
  • National Cancer Institute (CA097093)
  • National Institute of General Medical Sciences (P20GM075019, GM071023, GM075019, R01GM071023, GM07390)
  • National Institute of Allergy and Infectious Diseases (R01AI067952, AI051686, AI074967, R01AI074967, AI067952, R01AI051686)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
adeno-associated virus, recombinant AAV vectors, gene delivery, viral genome sequence, infectious clone, host-virus interaction, vector production
MeSH terms
Dependovirus, Genetic Vectors, Serotyping, Virus Replication, Gene Transfer Techniques

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