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Cited by 21Antifungal resistance and susceptibilityBacterial biofilms and quorum sensingFungal and yeast genetics research

Modeling of Fungal Biofilms Using a Rat Central Vein Catheter

Open access
Nett, Jeniel E., Marchillo, Karen, Andes, David R.·2012·Methods in Molecular Biology
DOI 10.1007/978-1-61779-539-8_40PMC PMC3341608
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Abstract

Candida frequently grows as a biofilm, or an adherent community of cells protected from both the host immune system and antimicrobial therapies. Biofilms represent the predominant mode of growth for many clinical infections, including those associated with placement of a medical device. Here, we describe a model for Candida biofilm infection of one important clinical niche, a venous catheter. This animal model system incorporates the anatomical site, immune components, and fluid dynamics of a patient venous catheter infection and can be used for study of biofilm formation, drug resistance, and gene expression.

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Crossref · 10.1007/978-1-61779-539-8_40

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  • No sex bias has been observed in the rat external jugular venous catheter biofilm model.

    “Experiments were conducted using male rats as no sex bias has been observed in this model, thus reducing the extraneous use of animals82,83.”

    From Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins (2025) · cites this paper as [83]

    The Introduction describes the rat jugular-vein catheter biofilm model, and Methods §3.4 describes animal care, but neither reports a comparison between male and female rats or addresses sex bias. Searches for sex bias, sex differences, and male rats found no passage supporting that claim. Page numbers were not available in our copy. (LLM, conf=0.76)

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Cites

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Research works printed but not linked: 12

  1. [1]Andes D Nett J Oschel P Albrecht R Marchillo K Pitula A Development and characterization of an in vivo central venous catheter Candida albicans biofilm model Infection and immunity 2004 72 6023 6031 15385506 PMC517581
  2. [2]Chandra J Kuhn DM Mukherjee PK Hoyer LL McCormick T Ghannoum MA Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance Journal of bacteriology 2001 183 5385 5394 11514524 PMC95423
  3. [3]Hawser SP Baillie GS Douglas LJ Production of extracellular matrix by Candida albicans biofilms Journal of medical microbiology 1998 47 253 256 9511830
  4. [4]Hogan DA Talking to themselves: autoregulation and quorum sensing in fungi Eukaryotic cell 2006 5 613 619 16607008 PMC1459667
  5. [5]Kojic EM Darouiche RO Candida infections of medical devices Clinical microbiology reviews 2004 17 255 267 15084500 PMC387407
  6. [6]Mukherjee PK Mohamed S Chandra J Kuhn D Liu S Antar OS Munyon R Mitchell AP Andes D Chance MR Rouabhia M Ghannoum MA Alcohol dehydrogenase restricts the ability of the pathogen Candida albicans to form a biofilm on catheter surfaces through an ethanol-based mechanism Infection and immunity 2006 74 3804 3816 16790752 PMC1489753
  7. [7]Nett J Andes D Candida albicans biofilm development, modeling a host-pathogen interaction Current opinion in microbiology 2006 9 340 345 16815078
  8. [8]Nett J Lincoln L Marchillo K Andes D Beta -1,3 glucan as a test for central venous catheter biofilm infection The Journal of infectious diseases 2007 195 1705 1712 17471441
  9. [11]Nikawa H Nishimura H Hamada T Kumagai H Samaranayake LP Effects of dietary sugars and, saliva and serum on Candida bioflim formation on acrylic surfaces Mycopathologia 1997 139 87 91 9549102
  10. [12]Nobile CJ Nett JE Hernday AD Homann OR Deneault JS Nantel A Andes DR Johnson AD Mitchell AP Biofilm matrix regulation by Candida albicans Zap1 PLoS biology 2009 7 e1000133 19529758 PMC2688839
  11. [13]Nobile CJ Schneider HA Nett JE Sheppard DC Filler SG Andes DR Mitchell AP Complementary adhesin function in C. albicans biofilm formation Curr Biol 2008 18 1017 1024 18635358 PMC2504253
  12. [14]Ramage G Vandewalle K Wickes BL Lopez-Ribot JL Characteristics of biofilm formation by Candida albicans Rev Iberoam Micol 2001 18 163 170 15496122

Cited by

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Authors
Nett, J. E., Marchillo, K., & Andes, D. R.
Affiliations
University of Wisconsin–Madison (US)
Corresponding author
Andes, David R.
ORCID
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2012
Volume
845
Pages
547-556
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-61779-539-8_40
PMC
PMC3341608
OpenAlex
W1665447717
Cited by
21
References
3
Citations by year
2025: 1 · 2024: 2 · 2023: 4 · 2020: 1 · 2019: 1 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 3
FWCI
7.95
Type
book chapter
Language
en
Open access
Licence
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (T32HL007899)
  • National Institute of Allergy and Infectious Diseases (R01AI073289)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
fungal biofilms, Candida, central venous catheters, catheter-related bloodstream infection, animal models, biofilm formation, drug resistance, gene expression, immune response, fluid dynamics
MeSH terms
Animals, Candida, Candidiasis, Catheterization, Central Venous, Equipment Contamination, Male, Models, Biological, Rats, Sprague-Dawley, Biofilms, Models, Animal, Rats

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