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SLIC: A Method for Sequence- and Ligation-Independent Cloning
Abstract
We describe here a method for sequence- and ligation-independent cloning (SLIC). SLIC uses an exonuclease, T4 DNA polymerase, to generate single-stranded DNA overhangs in insert and vector sequences. These fragments are then assembled in vitro and transformed into Escherichia coli to generate recombinant DNA of interest. SLIC inserts can also be generated by incomplete PCR (iPCR) or mixed PCR. As many as five inserts can be assembled in one reaction simultaneously with great efficiency using SLIC. SLIC circumvents sequence constraints for recombinant DNA using standard restriction enzyme-mediated cloning and previous ligation-independent cloning methods and provides a new approach for the efficient generation of recombinant DNA.
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Cited by
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Jiang, W., Zhao, X., Gabrieli, T., Lou, C., Ebenstein, Y., & Zhu, T. F. (2015) · Nature Communications · doi:10.1038/ncomms9101 · Cited by 267
Crystal structure of the human oxytocin receptor
Waltenspühl, Y., Schöppe, J., Ehrenmann, J., Kummer, L., & Plückthun, A. (2020) · Science Advances · doi:10.1126/sciadv.abb5419 · Cited by 95
Berla, B. M., Saha, R., Maranas, C. D., & Pakrasi, H. B. (2015) · Scientific Reports · doi:10.1038/srep14894 · Cited by 76
A genetic interaction analysis identifies cancer drivers that modify EGFR dependency
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Metadata
- Authors
- Li, M. Z., & Elledge, S. J.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2012
- Volume
- 852
- Pages
- 51-59
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W93462516
- Cited by
- 296
- References
- 0
- FWCI
- 27.46
- Type
- book chapter
- Language
- en
- Open access
- closed
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- Li, M. Z., & Elledge, S. J. (2012). SLIC: A Method for Sequence- and Ligation-Independent Cloning. Methods in Molecular Biology. https://doi.org/10.1007/978-1-61779-564-0_5
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