The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters
Open accessAbstract
The cloning of long DNA segments, especially those containing large gene clusters, is of particular importance to synthetic and chemical biology efforts for engineering organisms. While cloning has been a defining tool in molecular biology, the cloning of long genome segments has been challenging. Here we describe a technique that allows the targeted cloning of near-arbitrary, long bacterial genomic sequences of up to 100 kb to be accomplished in a single step. The target genome segment is excised from bacterial chromosomes in vitro by the RNA-guided Cas9 nuclease at two designated loci, and ligated to the cloning vector by Gibson assembly. This technique can be an effective molecular tool for the targeted cloning of large gene clusters that are often expensive to synthesize by gene synthesis or difficult to obtain directly by traditional PCR and restriction-enzyme-based methods.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
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Enzymatic assembly of DNA molecules up to several hundred kilobases
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CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
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DNA targeting specificity of RNA-guided Cas9 nucleases
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Genetic screens in human cells using the CRISPR-Cas9 system
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The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
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Cited by
Hao, Y., Li, Q., Fan, C., & Wang, F. (2021) · Small Structures · doi:10.1002/sstr.202000046 · Cited by 90
Metadata
- Authors
- Jiang, W., Zhao, X., Gabrieli, T., Lou, C., Ebenstein, Y., & Zhu, T. F.
- Venue
- Nature Communications
- Published
- 2015-09-01
- Volume
- 6
- Issue
- 1
- Pages
- 8101-8101
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1213974439
- Cited by
- 267
- References
- 34
- FWCI
- 6.95
- Type
- journal article
- Language
- en
- Open access
- gold
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- Jiang, W., Zhao, X., Gabrieli, T., Lou, C., Ebenstein, Y., & Zhu, T. F. (2015). Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nature Communications. https://doi.org/10.1038/ncomms9101
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