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Enhanced Angiogenesis and Astrocyte Activation by Ecdysterone Treatment in a Focal Cerebral Ischemia Rat Model1
Abstract
Background and purpose We reported previously that ecdysterone (EDS) improves neurologic function after experimental stroke. However, the underlying mechanism remained unclear. The present study was conducted to test whether ecdysterone improves neurologic function by enhancing astrocyte activation and angiogenesis after focal cerebral ischemia in rats. Methods Focal cerebral ischemia model was conducted by middle cerebral artery occlusion (MCAO). EDS was intraperitoneally injected at 20 mg kg1 daily for 7 days after MCAO. Neurologic recovery was assessed using the neurologic severity scores. Microvessel density and GFAP expression were detected with immunostaining and analyzed quantitatively with image system. Results Treatment with EDS significantly improved functional recovery, along with increases in density of cerebral microvessels and astrocyte activation. Microvessel density was significantly higher in EDS treated group than in ischemia control group at all time points, and reached a peak on day 14. EDS treated group had substantial increment in GFAP immunoreactive cells, darker staining color, more and longer nerve processes, higher GFAP expression and area of immunoreactive cells at each time point. Conclusion Our data suggest that EDS treatment enhanced angiogenesis and astrocyte activation which could contribute to functional recovery.
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In cerebral focal ischaemia of rat, 20E increased angiogenesis and astrocyte activity paralleled with increased microvascularization and formation of more and longer nerve endings.
“Similarly, in cerebral focal ischaemia of rat 20E, increased angiogenesis and astrocyte activity paralleled with increased microvascularization and formation of more and longer nerve endings [46].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [46]
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Research works printed but not linked: 15
- Li J, Qi H, Qi LW, Yi L, Li P. Stimultaneous determination of main phytoecdysones and triterpenoids in Radix Achyranthis Bidentatae by high-performance liquid chromatography with diode array-evaporative light scattering detectors and mass spectrometry. Anal Chim Acta. 2007;596(2):264–72. · DOI
- Luo CX, Zhang YQ, Chi L, Li L, Chen KN. Protective effect and mechanism of ecdysterone on injury of focal cerebral infarct in rats. Med J Nat Def South Chin. 2009;19(2):176–8.
- Li Y, Lu Z, Keogh CL, Yu SP, Wei L. Erythropoietin-induced neurovascular protection, angiogenesis, and cerebral blood flow restoration after focal ischemia in mice. J Cereb Blood Flow Metab. 2007;27:1043–54. · DOI
- Hoang S, Liauw J, Choi M, Choi M, Guzman RG, Steinberg GK. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J Cereb Blood Flow Metab. 2009;29(2):385–97. · DOI
- Jin J, Huang L, Xiang CQ, Li H, Wu X, Geng JM. Effcets of ecdysterone on collateral formation and expression of VEGF in rat AMI. Chin Pharmac Bul. 2000;16(4):459–61.
- Chen Z, Zhu G, Zhang JH, Liu Z, Tang WH, Feng H. Ecdysterone-sensitive smooth muscle cell proliferation stimulated by conditioned medium of endothelial cells cultured with bloody cerebrospinal fluid. Acta Neurochir Suppl. 2008;104:183–188. · DOI
- Tang WH, Chen Z, Liu Z, Zhang JH, Xi G, Feng H. The effect of Ecdysterone on cerebral vasospasm following Experimental Subarachnoid Hemorrhage in vitro and in vivo. Neurol Res. 2008;30(6):571–80. · DOI · Paper page
- Nedergaard M, Dirnagl U. Role of glial cells in cerebral ischemia. Glia 2005;50:281–6. · DOI
- Koehler RC, Gebremedhin D, Harder DR. Role of astrocytes in cerebrovascular regulation. J Appl Physiol. 2006;100(1):307–17. · DOI
- Panickar KS, Norenberg MD. Astrocytes in cerebral ischemic injury: morphological and general considerations. Glia 2005;50:287–98. · DOI
- Kirschenbaum SR, Higgins MR, Tveten M, Tolbert LP. 20-Hydroxyecdysone stimulates proliferation of glial cells in the developing brain of the moth Manduca sexta. J Neurobiol. 1995;28(2):234–47. · DOI
- Krun JM, Mani N, Rosenstein JM. Angiogenic and astroglial responses to vascular endothelial growth factor administration in adult rat brain. Neuroscience 2002;110:589–604. · DOI
- Haydon PG, Carmignoto G. Astrocyte control of synaptic transmission and neurovascular coupling. Physiol Rev. 2006;86(3):1009–31. · DOI
- Mahesh VB, Dhandapani KM, Brann DW. Role of astrocytes in reproduction and neurprotection. Mol Cell Endocrinol. 2006;246:1–9. · DOI
- Lathia JD, Chigurupati S, Thundyil J, Selvaraj PK, Mughal MR, Woodruff TM, et al. Pivotal role for beta-1 integrin in neurovascular remodeling after ischemic stroke. Exp Neurol. 2010;221(1):107–114. · DOI
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Dinan, L., Dioh, W., Veillet, S., & Lafont, R. (2021) · Biomedicines · doi:10.3390/biomedicines9050492 · Cited by 99
Zádor, E. (2025) · Cells · doi:10.3390/cells14060431 · Cited by 9
Metadata
- Authors
- Luo, C., Yi, B., Fan, W., Chen, K., Gui, L., Chen, Z., Li, L., Feng, H., & Chi, L.
- Affiliations
- Army Medical University (CN)
- Corresponding author
- Feng, Hua
- Chi, Luxiang
- Venue
- Early Brain Injury or Cerebral Vasospasm
- Publisher
- Springer Vienna
- Published
- 2011
- Volume
- 110
- Issue
- Pt 1
- Pages
- 151-155
- OpenAlex
- W1543423686
- Cited by
- 14
- References
- 0
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 1
- FWCI
- 1.08
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Springer eBooks (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroprotection in Cerebral Ischemia
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Clinical Neurology › Acute Ischemic Stroke Management
- Keywords
- 20-hydroxyecdysone, angiogenesis, astrocyte activation, focal cerebral ischemia, stroke, middle cerebral artery occlusion, neurological recovery, microvessel density, glial fibrillary acidic protein, functional recovery
- MeSH terms
- Analysis of Variance, Animals, Astrocytes, Brain Ischemia, Disease Models, Animal, Ecdysterone, Factor VIII, Glial Fibrillary Acidic Protein, Severity of Illness Index, Time Factors, Rats, Sprague-Dawley, Neovascularization, Physiologic, Recovery of Function, Rats, Microvessels
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