The effect of ecdysterone on cerebral vasospasm following experimental subarachnoid hemorrhage in vitro and in vivo
Open accessAbstract
OBJECTIVES: Cerebral vasospasm has been the dreaded complication of ruptured intracranial aneurysms. Worldwide effort has led to many promising experimental treatments but none was confirmed to be effective in clinical trials. Ecdysterone is an insect steroid hormone. Our previous study showed that ecdysterone might prevent cerebral vasospasm in vitro. Even after all these works, rare attempts have been made to test the effect of ecdysterone on vascular adventitial fibroblast (VAF) proliferation, a process known to play an important role in various pathogenic vascular conditions. Thus, we tested the hypothesis that ecdysterone could affect VAF characteristics and have an effect on SAH induced cerebral vasospasm. METHODS: OxyHb of 100 microM was used in the in vitro study to mimic the clinical situation. The effect of OxyHb on the cell proliferation and migration of cultured aortic smooth muscle cells was investigated. In the in vivo study, 20 rabbits were equally divided into four groups: control group, SAH group, SAH/nimodipine group and SAH/ecdysterone group. Changes in neurological function and cerebral angiograms were observed after SAH. RESULTS: OxyHb increased the proliferation of vascular adventitial fibroblasts at 24 hours. Ecdysterone co-treatment was apparently similar to the suppression of proliferation. Cell cycle analysis indicated that ecdysterone inhibited the progression of vascular adventitial fibroblasts from G1 to S. The results of the migration assay showed that 100 microM OxyHb obviously prompted vascular adventitial fibroblast migration and that ecdysterone would attenuate this effect. In the SAH/nimodipine and SAH/ecdysterone groups, neurological deficit, cerebral vasospasm and structural changes in basilar artery were alleviated with nimodipine or ecdysterone treatment. CONCLUSION: Ecdysterone could affect vascular adventitial fibroblast characteristics and attenuate vasospasm after SAH.
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Ecdysterone attenuated vasospasm and alleviated neurological deficits after subarachnoid haemorrhage in rabbits.
“Ecdysterone could attenuate vasospasm and alleviate neurological deficits that happen as a consequence of subarachnoid haemorrhage in rabbits [44].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [44]
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“Ecdysterone could attenuate vasospasm and alleviate neurological deficits that happen as a consequence of subarachnoid haemorrhage in rabbits [44].”
- Authors
- Tang, W.-H., Chen, Z., Liu, Z., Zhang, J. H., Xi, G., & Feng, H.
- Affiliations
- Army Medical University (CN)
- Loma Linda University Medical Center (US)
- University of Michigan (US)
- Venue
- Neurological Research
- Publisher
- Informa UK Limited
- Published
- 2008-07-07
- Volume
- 30
- Issue
- 6
- Pages
- 571-580
- ISSN
- 0161-6412 (ISSN-L)
- 1743-1328
- PMC
- PMC2706528
- OpenAlex
- W2048629241
- Cited by
- 15
- References
- 26
- Citations by year
- 2025: 1 · 2022: 2 · 2014: 1 · 2013: 2 · 2012: 4
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Neurological Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS045694)
- Topics
- Health Sciences › Medicine › Neurology › Intracranial Aneurysms: Treatment and Complications
- Health Sciences › Medicine › Neurology › Intracerebral and Subarachnoid Hemorrhage Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Takotsubo Cardiomyopathy and Associated Phenomena
- Keywords
- 20-hydroxyecdysone, cerebral vasospasm, subarachnoid hemorrhage, vascular adventitial fibroblasts, cell proliferation, cell migration, cell cycle, oxyhemoglobin, aortic smooth muscle cells, nimodipine, basilar artery, ruptured intracranial aneurysm, neurological deficit
- MeSH terms
- Animals, Aorta, Behavior, Animal, Cell Cycle, Cell Movement, Cells, Cultured, Cerebral Angiography, Disease Models, Animal, Ecdysterone, Female, Fibroblasts, Male, Oxyhemoglobins, Rabbits, Subarachnoid Hemorrhage, Time Factors, Rats, Sprague-Dawley, Ultrasonography, Doppler, Transcranial, Vasospasm, Intracranial, Microscopy, Electron, Transmission, Rats, In Vitro Techniques
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