Effects of CO2 and Osmolality on Hybridoma Cells: Growth, Metabolism and Monoclonal Antibody Production
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Supra-physiological osmolarity can directly affect cell membrane tension and volume, but can also moderate how cells respond to other environmental parameters.
“Supra-physiological osmolarity can directly affect cell membrane tension and volume (Pedersen et al., 2013), but can also moderate how cells respond to other environmental parameters (Dezengotita et al., 1998).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Dezengotita et al., 1998
Hybridoma cells exposed to elevated CO2 conditions exhibited reduced growth rates when osmolarity was held constant at 361 mOsm kg−1, but cell growth rates further declined by 30% when medium osmolality was 415 mOsm kg−1.
“For instance, hybridoma cells exposed to elevated CO2 conditions exhibited reduced growth rates when osmolarity was held constant at 361 mOsm kg−1, but cell growth rates further declined by 30% when medium osmolality was 415 mOsm kg−1 (Dezengotita et al., 1998).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Dezengotita et al., 1998
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- Authors
- deZengotita, V. M., Kimura, R., & Miller, W. M.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Miller, William M.
- ORCID
- Miller, William M.
- Venue
- Current Applications of Cell Culture Engineering
- Publisher
- Springer Netherlands
- Published
- 1998
- Pages
- 213-227
- ISSN
- 1386-2928 (ISSN-L)
- OpenAlex
- W4252006125
- Cited by
- 11
- References
- 19
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2013: 1
- FWCI
- 0.00
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Current applications of cell culture engineering (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Keywords
- hybridoma, monoclonal antibody production, cell growth inhibition, cell death rate, glucose utilization, lactic acid production, glycolysis, continuous culture, oxygen consumption
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