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Cited by 18Diet, Metabolism, and DiseaseMetabolism, Diabetes, and CancerLipid metabolism and biosynthesis

Enzymes of carbohydrate metabolism in young and adult rats fed diets differing in fat and carbohydrate

Brooks, StephenP.J., Lampi, BrianJ.·1996·Molecular and Cellular Biochemistry
DOI 10.1007/bf00226063
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Abstract

Glycogen content as well as glycolytic, gluconeogenic and fatty acid synthesis enzyme activities were monitored in young and adult male rats fed diets differing in fat content: 11% (low), 22% (medium) and 42% (high) of total energy from fat. The results showed significant differences in the responses of young and adult rats to changes in dietary fat and carbohydrate. In young animals, increasing dietary fat decreased total liver glycogen phosphorylase (GP), pyruvate kinase (PK), glycerol 3-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase, malic enzyme (ME), ATP-citrate lyase (ATP-CL) and fatty acid synthase (FAS). Increasing dietary fat also affected enzyme levels in other tissues: hexokinase (HK) and pyruvate dehydrogenase (PDH) activities decreased whereas skeletal muscle PK activity increased. The pattern of enzyme changes was similar in livers of fed adults with the exception that liver GP was not affected by dietary manipulations. Overnight food deprivation decreased liver glucokinase (GK), ME, ATP-CL, and FAS activities and increased liver phosphoenolpyruvate carboxykinase (PEPCK) and phosphofructokinase in both young and adult animals. In young animals, food deprivation also: (i) reduced liver GK and PK, (ii) increased kidney PEPCK, (iii) decreased muscle PEPCK and (iv) decreased kidney PDH. Food-deprived adults had increased skeletal muscle PEPCK and kidney glycogen synthetase as well as decreased kidney PEPCK muscle GP activity. These differences suggest that young animals are somewhat more responsive to changes in dietary manipulations. They also show that overnight food restriction causes a more profound metabolic re-organization in younger than in older animals.

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Crossref · 10.1007/bf00226063

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  • Impaired metabolic flexibility with regard to both supply and demand perturbations has been reported in old age.

    “Impaired metabolic flexibility with regard to both supply and demand perturbations has been reported in old age [65, 80, 89, 92]”

    From Targeting Intramyocellular Lipids to Improve Aging Muscle Function (2025) · cites this paper as [92]

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Authors
Brooks, S. J., & Lampi, B.
Affiliations
Health Canada (CA)
Venue
Molecular and Cellular Biochemistry
Publisher
Springer Science and Business Media LLC
Published
1996-06
Volume
159
Issue
1
Pages
55-63
ISSN
  • 0300-8177 (ISSN-L)
  • 1573-4919
DOI
10.1007/bf00226063
OpenAlex
W1981595878
Cited by
18
References
45
Citations by year
2026: 1 · 2025: 1
FWCI
1.61
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Molecular and Cellular Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
carbohydrate metabolism, dietary fat, dietary carbohydrates, glycogen phosphorylase, pyruvate kinase, gluconeogenesis, fatty acid synthesis, ATP citrate lyase, fatty acid synthase, food deprivation, aging, liver, skeletal muscle, kidney, phosphoenolpyruvate carboxykinase, phosphofructokinase, malic enzyme, glucokinase, glycogen synthase, pyruvate dehydrogenase, hexokinase
MeSH terms
Age Factors, Animals, Dietary Carbohydrates, Dietary Fats, Enzymes, Fatty Acids, Food Deprivation, Gluconeogenesis, Glycogen, Kidney, Liver, Male, Plasma, Rats, Sprague-Dawley, Muscle, Skeletal, Rats

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