Lengths of transcribed rDNA repeating units in spermatocytes of drosophila hydei: only genes without an intervening sequence are expressed
Abstract
In Drosophila hydei a 5 kb long intervening sequence (ivs) which is limited to only one of the three nucleolus organizers occurs in the 28S coding region of the rDNA (Kunz and Glätzer, 1979). This raises the possibility to study transcription of repeated genes with long intervening sequences. Three genotypes of males were studied each having only one defined nucleolus organizer. Spermatocytes of single males were spread in a solution of low salt and examined by electron microscopy. The length distributions of the repeating units, matrix units, and the apparent spacer units did not reveal two classes with a 5 kb length difference. The data suggest that in D. hydei the 5 kb long intervening sequence is not transcribed and that, in fact, the entire gene which contains an intervening sequence remains silent in primary spermatocytes. There is evidence that genes with an intervening sequence are not intermingled with "normal" genes at random.
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Electron microscopy showed that the low level of R2 transcripts is mainly due to transcriptional repression of the entire rDNA units that contain insertions.
“Electron microscopy using the ‘Miller spreading technique’ (6) showed that the low level of R2 transcripts is mainly due to the transcriptional repression of the entire rDNA units, which contain insertions (51,52).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [52]
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Jamrich, M., & Miller, O. L. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb02008.x · Cited by 66 · see citing works
Fefelova, E. A., Pleshakova, I. M., Mikhaleva, E. A., Pirogov, S. A., Poltorachenko, V. A., Abramov, Y. A., Romashin, D. D., Shatskikh, A. S., Blokh, R. S., Gvozdev, V. A., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1276 · Cited by 15 · see citing works
- Authors
- Gl�tzer, K. H.
- Venue
- Chromosoma
- Published
- 1979-11
- Volume
- 75
- Issue
- 2
- Pages
- 161-175
- ISSN
- 0009-5915 (ISSN-L)
- 1432-0886
- OpenAlex
- W307617521
- Cited by
- 32
- References
- 0
- Citations by year
- 2021: 1
- FWCI
- 8.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Chromosoma (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- Drosophila hydei, rDNA transcription, introns, 28S rRNA gene, nucleolus organizer, spermatocytes, gene silencing
- MeSH terms
- Animals, Base Sequence, DNA, Drosophila, Genes, Male, Nucleolus Organizer Region, RNA, Heterogeneous Nuclear, RNA, Ribosomal, Spermatocytes, Transcription, Genetic
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