Codon specificity of starvation induced misreading
Abstract
Mistranslated derivatives of the coat protein of the bacteriophage MS2 were isolated from infected cells starved for asparagine. This protein contains a high level of lysine for asparagine substitutions. By peptide analysis and amino acid sequencing we show that there is a six-fold greater frequency of errors at AAU codons than at AAC codons. This ratio is the same as that found in unstarved cells where the overall error frequency is 100-fold less. We also demonstrate that, at least for AAC codons, context affects error frequency.
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AAC and AAU differ in misreading rate by tRNALys, leading to Asn replaced by Lys.
“However, AAC and AAU also differ in misreading rate, in particular by tRNALys, which ideally should decode only AAA and AAG codons but does misread AAC and AAU, leading to Asn replaced by Lys.”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [38]
The misreading error rate is six times greater for AAU than for AAC, with the error ratio consistently maintained under Asn-starved and non-starved conditions and with Streptomycin.
“This misreading error rate is six times greater for AAU than for AAC, with the error ratio consistently maintained in different experimental settings, e.g., under both Asn-starved and non-starved conditions [38], or with Streptomycin used to inhibit translation [39].”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [38]
Cites
The frequency of errors in protein biosynthesis
Loftfield, R. B., & Vanderjagt, D. (1972) · Biochemical Journal · doi:10.1042/bj1281353 · Cited by 399 · see citing works
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Nonrandom utilization of codon pairs in Escherichia coli.
Gutman, G. A., & Hatfield, G. W. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.10.3699 · Cited by 261 · see citing works
Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines
Xia, X. (2021) · Vaccines · doi:10.3390/vaccines9070734 · Cited by 201 · see citing works
- Authors
- Johnston, T. C., Borgia, P. T., & Parker, J.
- Affiliations
- Southern Illinois University Carbondale (US)
- SIU Medicine (US)
- ORCID
- Johnston, Timothy C.
- Venue
- Molecular and General Genetics MGG
- Published
- 1984-07
- Volume
- 195
- Issue
- 3
- Pages
- 459-465
- ISSN
- 0026-8925 (ISSN-L)
- 1432-1874
- OpenAlex
- W1987740343
- Cited by
- 47
- References
- 1
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2018: 6 · 2017: 2 · 2015: 1
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and General Genetics MGG (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- codon specificity, translation errors, codon context, error rate
- MeSH terms
- Amino Acid Sequence, Asparagine, Capsid, Codon, Coliphages, RNA, Messenger, Protein Biosynthesis, RNA-Binding Proteins, Capsid Proteins
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