The frequency of errors in protein biosynthesis
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Every protein molecule consists basically of a
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Error frequencies of 0.01-0.1% per amino acid have been estimated for misincorporating structurally similar amino acids in vivo.
“Error frequencies of 0.01-0.1% per amino acid (AA) have been estimated for misincorporating structurally similar amino acids in vivo 20 .”
From How many human proteoforms are there? (2018) · cites this paper as [20]
The rabbit haemoglobin peptide discussion (page not specified in our copy) estimates 2–6 substitutions per 10,000 sites, or 0.02–0.06%, within the claimed range. But the authors say it remains uncertain whether the measured valine incorporation is largely substitution for isoleucine or whether other structurally similar amino-acid pairs have comparable error frequencies. The experiment used incubated rabbit reticulocytes, rather than measuring errors in vivo. (LLM, conf=0.87)
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- Authors
- Loftfield, R. B., & Vanderjagt, D.
- Affiliations
- University of New Mexico (US)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1972-08-01
- Volume
- 128
- Issue
- 5
- Pages
- 1353-1356
- ISSN
- 0306-3283 (ISSN-L)
- PMC
- PMC1174024
- OpenAlex
- W1923250545
- Cited by
- 399
- References
- 3
- Citations by year
- 2026: 7 · 2025: 3 · 2024: 6 · 2023: 5 · 2022: 8 · 2021: 5 · 2020: 5 · 2019: 8 · 2018: 9 · 2017: 11 · 2016: 12 · 2015: 7 · 2014: 15 · 2013: 8 · 2012: 7
- FWCI
- 2.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- protein synthesis, error rate, translation errors, protein synthesis fidelity, aminoacyl-tRNA
- MeSH terms
- Amino Acid Sequence, Animals, DNA, Genetic Code, Hemoglobins, Hemoglobins, Abnormal, Humans, Rabbits, Reticulocytes, RNA, Transcription, Genetic, Protein Biosynthesis, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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