Effect of adrenal steroids on insulin release from cultured rat islets of Langerhans
Open accessAbstract
The effect of additions to the culture medium of some natural or synthetic corticosteroid hormones was studied in cultured rat islets of Langerhans. The steroids decreased glucose-induced insulin release. The extent of inhibition by dexamethasone was 18-55%, prednisolone 23%, hydrocortisone 21% and aldosterone 18%. None of them affected the basal secretion of insulin or had any effect on diameter or insulin content of the islet. The inhibitory action of dexamethasone on insulin release was observed in the range 63 nmol/l to 6.3 mumol/l. At 6.3 mumol/l during two h, dexamethasone (a) inhibited insulin response to glucose concentrations above 5 mmol/l (b) caused a delay in the first phase and markedly reduced the second phase of insulin release of perifused islets, and (c) decreased the incorporation of [H3]-leucine into total islet proteins without affecting [H3]-leucine-incorporation into insulin plus proinsulin. It is suggested that steroids, by directly acting on the islets of Langerhans, may modulate the insulin-release response to secretagogues.
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Aldosterone reduces insulin secretion through direct effects on the β-cell.
“Aldosterone reduces insulin secretion by direct effects on the β-cell [36-38].”
From Insulin sensitivity and pancreatic β-cell function in patients with primary aldosteronism (2021) · cites this paper as [37]
The Results and Discussion report that aldosterone directly applied to cultured rat islets reduced glucose-stimulated insulin release. The experiments used whole islets, however, and do not establish that aldosterone acted directly on β-cells rather than elsewhere within the islets. (LLM, conf=0.83)
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- Authors
- Pierluissi, J., Navas, F. O., & Ashcroft, S. J. H.
- Affiliations
- Universidad Central de Venezuela (VE)
- John Radcliffe Hospital (GB)
- Venue
- Diabetologia
- Published
- 1986-02
- Volume
- 29
- Issue
- 2
- Pages
- 119-121
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- OpenAlex
- W1985476778
- Semantic Scholar
- de7abf4a6f30107b3a75d704c8b491597084171b
- Cited by
- 66
- References
- 10
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Diabetologia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional para Investigaciones Científicas y Tecnológicas
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- adrenal steroids, insulin secretion, pancreatic islets, dexamethasone, prednisolone, cortisol, aldosterone, corticosteroids, glucose-induced insulin release, first-phase insulin response, second-phase insulin response, protein synthesis, proinsulin, secretagogues
- MeSH terms
- Insulin Secretion, Aldosterone, Animals, Cells, Cultured, Dexamethasone, Hydrocortisone, Insulin, Islets of Langerhans, Kinetics, Male, Prednisolone, Rats, Inbred Strains, Rats
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