The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Microbes, enzymes and genes involved in dichloromethane utilization
Abstract
Dichloromethane (DCM) is efficiently utilized as a carbon and energy source by aerobic, Gram-negative, facultative methylotrophic bacteria. It also serves as a sole carbon and energy source for a nitrate-respiring Hyphomicrobium sp. and for a strictly anaerobic co-culture of a DCM-fermenting bacterium and an acetogen. The first step of DCM utilization by methylotrophs is catalyzed by DCM dehalogenase which, in a glutathione-dependent substitution reaction, forms inorganic chloride and S-chloromethyl glutathione. This unstable intermediate decomposes to glutathione, inorganic chloride and formaldehyde, a central metabolite of methylotrophic growth. Genetic studies on DCM utilization are beginning to shed some light on questions pertaining to the evolution of DCM dehalogenases and on the regulation of DCM dehalogenase expression. DCM dehalogenase belongs to the glutathione S-transferase supergene family. Analysis of the amino acid sequences of two bacterial DCM dehalogenases reveals 56% identity, and comparison of these sequences to those of glutathione S-transferases indicates a closer relationship to class Theta eukaryotic glutathione S-transferases than to a number of bacterial glutathione S-transferases whose sequences have recently become available. dcmA, the structural gene of the highly substrate-inducible DCM dehalogenase, is carried in most DCM utilizing methylotrophs on large plasmids. In Methylobacterium sp. DM4 its expression is governed by dcmR, a regulatory gene l…
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“It is well accepted that dehalogenases reactive with organochlorine and organobromine compounds have an ancient evolutionary origin and processed natural products prior to industrialization”
From Nothing lasts forever: understanding microbial biodegradation of polyfluorinated compounds and perfluorinated alkyl substances (2022) · cites this paper as Leisinger et al., 1994
Citations
Cites
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Research works printed but not linked: 10
- Bader R & Leisinger T (1993) Characterization ofdcmA inMethylophilus sp. DM11, the plasmid encoded structural gene of dichloromethane dehalogenase (Abstract). BioEngineering 9:58, P261
- Bader R (1994) Das Strukturgen der Dichlormethan Dehalogenase/Glutathion S-Transferase ausMethylophilus sp. Stamm DM11. Ph.D. Thesis. Federal Institute of Technology, Zürich
- Frank S (1993) Abbau von Dichlormethan durchHyphomicrobium DM2 unter denitrifizierenden Bedingungen. Diploma Thesis, Swiss Federal Institute of Technology, Zürich
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- Green PN (1993) Overview of the current state of methylotroph taxonomy. In: Murrell JC & Kelly DP (Eds) Microbial Growth on C1 Compounds (pp 253?265). Intercept Ltd, Andover, UK
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Cited by
Wackett, L. P. (2022) · Microbial Biotechnology · doi:10.1111/1751-7915.13928 · Cited by 165
Metadata
- Authors
- Leisinger, T., Bader, R., Hermann, R., Schmid-Appert, M., & Vuilleumier, S.
- Venue
- Biodegradation
- Published
- 1994-12
- Volume
- 5
- Issue
- 3-4
- Pages
- 237-248
- ISSN
- 0923-9820
- 1572-9729
- Cited by
- 73
- References
- 59
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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