Characterization of an immunodominantGiardia lamblia protein antigen related to alpha giardin
Abstract
The trophozoites of Giardia lamblia possess several protein antigens, predominant among them a protein of approximately 32,000 Da. In the present study, we used monospecific antibodies that recognize this protein to demonstrate its presence on a variety of G. lamblia isolates from human and animal sources. Immune electron microscopy was used to localize 32-kDa antigen on the trophozoite membrane and disk. Immunofluorescent assays employing monospecific antibodies confirmed the presence of 32-kDa antigen on the membrane and disk and its absence on flagella or nuclei. The N-terminal 17 amino acids of the 32-kDa antigen are identical to alpha-1-giardin, a protein component of microribbons on the ventral disk. These results suggest that the 32-kDa immunodominant trophozoite antigen is alpha-1-giardin.
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Alpha-giardins were originally discovered as prominent proteins of the structurally complex Giardia intestinalis cytoskeleton and found to be immunodominant during human G. intestinalis infection.
“The alpha-giardins were originally discovered as prominent proteins of the structurally complex Giardia intestinalis cytoskeleton and found to be immunodominant during human G. intestinalis infection (5, 6).”
From Comparative Cell Biology and Evolution of Annexins in Diplomonads (2016) · cites this paper as [5]
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- Authors
- Wenman, W. M., Meuser, R. U., Nyugen, Q., Kilani, R. T., El-Shewy, K., & Sherburne, R.
- Affiliations
- University of Alberta (CA)
- ORCID
- Kilani, Ruhangiz T.
- Venue
- Parasitology Research
- Published
- 1993
- Volume
- 79
- Issue
- 7
- Pages
- 587-592
- ISSN
- 0044-3255 (ISSN-L)
- 1432-1955
- OpenAlex
- W1975479122
- Cited by
- 23
- References
- 31
- Citations by year
- 2023: 1 · 2020: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2012: 1
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Parasitology Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
- Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
- Keywords
- Giardia lamblia, microribbons, immunodominant antigens, trophozoite ultrastructure, antigen localization
- MeSH terms
- Amino Acid Sequence, Animals, Antibodies, Protozoan, Antibody Specificity, Antigens, Protozoan, Cell Movement, Cytoskeletal Proteins, Fluorescent Antibody Technique, Glycosylation, Molecular Sequence Data, Protein Processing, Post-Translational, Tunicamycin, Protozoan Proteins, Immunodominant Epitopes, Microscopy, Immunoelectron, Giardia lamblia, Sequence Analysis
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