The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci
Abstract
We have examined the performance and reproducibility of an automated DNA profiling system which is based on the multiplex amplification of 4 tetrameric STR loci-HUMVWFA31/A. HUMTH01, HUMF13A1 and HUMFES/FPS. The system was able to type 100 pg of purified, undegraded, genomic DNA. At lower concentrations of DNA (below 100 pg), allelec drop-out occurred due to stochastic differences in allele copy number. Minor variation of individual PCR reagent concentrations or cycling temperatures did not result in a significant effect on the efficiency of amplification of any of the 4 loci in the quadruplex system. More substantial variation of reagent concentrations or cycling temperatures outside the optimum range of the system resulted in a reduction or complete loss of signal for one or more loci. This was also observed at high ionic strength or extreme pH. However, under all reagent concentrations and conditions studied, no artefact bands that could potentially result in the mistyping of a sample were apparent within the read region (130-240 bases) of the gel. Evaluation of both native and denaturing polyacrylamide gels revealed that, although native gels displayed faster run times, the sizing precision of such gels for certain STR loci was lower than that of denaturing gels. Also, artefact bands may be present within the read region of native gels. In conclusion the quadruplex amplification system described, coupled with automated fluorescence-based detection on denaturing polyacrylamide gels, appeared to be a robust and reliable system for individual identification.
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“This doubt perhaps played a role in the Russian Orthodox Church's refusal to accept the identification of the remains in 1998 [5].”
From The identification of the Romanovs: Can we (finally) put the controversies to rest? (2011) · cites this paper as [5]
Citations
Cites
PCR protocols — A guide to methods and applications
U.G. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90252-7 · Cited by 11,702
DNA typing and genetic mapping with trimeric and tetrameric tandem repeats.
Edwards, A., Civitello, A. B., Hammond, H. A., & Caskey, C. T. (1991) · Europe PMC (PubMed Central) · Cited by 1,147
Clark, J. M. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.20.9677 · Cited by 843
Innis, M. A., Myambo, K. B., Gelfand, D. H., & Brow, M. A. D. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.24.9436 · Cited by 776
Identification of the remains of the Romanov family by DNA analysis
Gill, P., Ivanov, P. L., Kimpton, C., Piercy, R., Benson, N., Tully, G., Evett, I., Hagelberg, E., & Sullivan, K. (1994) · Nature Genetics · doi:10.1038/ng0294-130 · Cited by 693
Self-citationSAMPLE PREPARATION FROM BLOOD, CELLS, AND OTHER FLUIDS
Kawasaki, E. S. (1990) · PCR Protocols · doi:10.1016/b978-0-12-372180-8.50022-6 · Cited by 421
Automated DNA profiling employing multiplex amplification of short tandem repeat loci.Preprint
Kimpton, C. P., Gill, P., Walton, A., Urquhart, A., Millican, E. S., & Adams, M. (1993) · Genome Research · doi:10.1101/gr.3.1.13 · Cited by 395
Self-citationIdentification of the skeletal remains of a murder victim by DNA analysis
Hagelberg, E., Gray, I. C., & Jeffreys, A. J. (1991) · Nature · doi:10.1038/352427a0 · Cited by 315
A further tetranucleotide repeat polymorphism in the vWF gene
Kimpton, C., Walton, A., & Gill, P. (1992) · Human Molecular Genetics · doi:10.1093/hmg/1.4.287 · Cited by 304
Self-citationApplication of automated DNA sizing technology for genotyping microsatellite loci
Ziegle, J. S., Su, Y., Corcoran, K. P., Nie, L., Eric Mayrand, P., Hoff, L. B., McBride, L. J., Kronick, M. N., & Diehl, S. R. (1992) · Genomics · doi:10.1016/s0888-7543(05)80126-0 · Cited by 276
Cited by
Genetics and Genomics of Core Short Tandem Repeat Loci Used in Human Identity Testing
Butler, J. M. (2006) · Journal of Forensic Sciences · doi:10.1111/j.1556-4029.2006.00046.x · Cited by 686
Mystery Solved: The Identification of the Two Missing Romanov Children Using DNA Analysis
Coble, M. D., Loreille, O. M., Wadhams, M. J., Edson, S. M., Maynard, K., Meyer, C. E., Niederstätter, H., Berger, C., Berger, B., Falsetti, A. B., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004838 · Cited by 186
Self-citationDeforce, D. L. D., Millecamps, R. E. M., Van Hoofstat, D., & Van den Eeckhout, E. G. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)00394-4 · Cited by 33
Ancient DNA-typing approaches for the determination of kinship in a disturbed collective burial site
Schultes, T., Hummel, S., & Herrmann, B. (2000) · Anthropologischer Anzeiger · doi:10.1127/anthranz/58/2000/37 · Cited by 28
The identification of the Romanovs: Can we (finally) put the controversies to rest?
Coble, M. D. (2011) · Investigative Genetics · doi:10.1186/2041-2223-2-20 · Cited by 13
“The first four of these markers were used to create the “quadruplex,” one of the first multiplex STR assay kits [8].”
Method
Metadata
- Authors
- Kimpton, C., Fisher, D., Watson, S., Adams, M., Urquhart, A., Lygo, J., & Gill, P.
- Affiliations
- Armed Forces Institute of Pathology (US)
- The Priory Hospital (GB)
- Venue
- International Journal of Legal Medicine
- Published
- 1994-11
- Volume
- 106
- Issue
- 6
- Pages
- 302-311
- ISSN
- 0044-3433 (ISSN-L)
- 0937-9827
- 1437-1596
- OpenAlex
- W2026896735
- Cited by
- 183
- References
- 27
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 8 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 8 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 2
- FWCI
- 11.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Legal Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- DNA fingerprinting, multiplex PCR, HUMTH01, allele dropout, PCR artefacts, denaturing polyacrylamide gel electrophoresis, native polyacrylamide gel electrophoresis, individual identification, forensic genetics
- MeSH terms
- Alleles, Base Sequence, DNA, Electrophoresis, Polyacrylamide Gel, Evaluation Studies as Topic, Forensic Medicine, Humans, Hydrogen-Ion Concentration, Molecular Sequence Data, Multiphasic Screening, Osmolar Concentration, Repetitive Sequences, Nucleic Acid, Signal Processing, Computer-Assisted, Stochastic Processes, Temperature, Time Factors, Reproducibility of Results, Genome, Human, Bias, Polymerase Chain Reaction, DNA Fingerprinting
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
