Homospecific activity (activity per enzyme protein) of tyrosine hydroxylase increases in parkinsonian brain
Abstract
Tyrosine hydroxylase (TH) contents in the caudate nucleus, putamen, and substantia nigra from control and parkinsonism brains were measured for the first time by a sandwich enzyme immunoassay. Both the TH protein content and TH activity (Vmax) were decreased in parallel in the parkinsonian brains as compared with those of the control brains. In contrast, TH "homospecific activity" (activity per enzyme protein) was significantly increased in the parkinsonian brains. The results indicate that the decrease of TH activity in parkinsonian brains is due to the decrease of TH protein content as a result of cell death. The increase in the "homospecific activity" of residual TH in parkinsonian brain suggests such molecular changes in TH molecules as result in a compensatory increase in TH activity.
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In PD, the decrease in total TH protein level in the striatum is greater than the decrease in total TH enzyme activity.
“Mogi et al. found that a decrease in total TH protein level in the striatum was greater than that in the total enzyme activity, as assessed by enzyme immunoassay [69].”
From Degradation of Tyrosine Hydroxylase by the Ubiquitin-Proteasome System in the Pathogenesis of Parkinson’s Disease and Dopa-Responsive Dystonia (2020) · cites this paper as [69]
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Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15567871
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15568021
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15568048
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15568006
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15567961
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15567889
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15568078
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15567880
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15568066
Houser, M. C., Caudle, W. M., Chang, J., Kannarkat, G. T., Yang, Y., Kelly, S. D., Oliver, D. L., Joers, V. L., Shannon, K. M., Keshavarzian, A., et al. (2021) · Figshare · doi:10.6084/m9.figshare.15567985
- Authors
- Mogi, M., Harada, M., Kiuchi, K., Kojima, K., Kondo, T., Narabayashi, H., Rausch, D., Riederer, P., Jellinger, K., & Nagatsu, T.
- Affiliations
- Matsumoto Dental University (JP)
- Nagoya University (JP)
- Food and Drug Safety Center (JP)
- Juntendo University (JP)
- University of Veterinary Medicine Vienna (AT)
- University of Würzburg (DE)
- Venue
- Journal of Neural Transmission
- Published
- 1988-02
- Volume
- 72
- Issue
- 1
- Pages
- 77-82
- ISSN
- 0300-9564 (ISSN-L)
- 1435-1463
- OpenAlex
- W2013700933
- Cited by
- 101
- References
- 14
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 16 · 2020: 6 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 1 · 2012: 1
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Neural Transmission (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Topics
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- tyrosine hydroxylase, Parkinson's disease, enzyme protein content, caudate nucleus, putamen, substantia nigra, dopaminergic neuron loss
- MeSH terms
- Brain Chemistry, Caudate Nucleus, Corpus Striatum, Humans, Immunoenzyme Techniques, Parkinson Disease, Putamen, Substantia Nigra, Tyrosine 3-Monooxygenase
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