Cited by 44Animal Virus Infections StudiesVirology and Viral DiseasesCellular transport and secretion

Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells

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Chasey, D., Alexander, D. J.·1976·Archives of Virology

Abstract

Primary chick kidney cells were infected with avian infectious bronchitis virus (IBV) and examined by electron microscopy. Virus particles entered the cells by viropexis and distinction could be made between engulfment by cell processes (phagocytosis) and entry by micropinocytosis in coated transport vesicles. Virus maturation occurred by budding into either the cisternae of the endoplasmic reticulum or cytoplasmic vacuoles, and evidence was obtained to suggest that the viral surface projections could be attached during the budding process. Late in infection large numbers of virus particles were present, mainly in cytoplasmic vacuoles, and the majority were released by cell lysis. Release by fusion of vacuoles with the plasma membrane was also observed, and individual virions could be transported from the endoplasmic reticulum to the surface within coated vesicles.

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Crossref · 10.1007/bf01317869

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15 of 15 references verified0 citation errors · 0 not checkable

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Authors
Chasey, D., & Alexander, D. J.
Venue
Archives of Virology
Published
1976-03
Volume
52
Issue
1-2
Pages
101-111
ISSN
  • 0003-9012 (ISSN-L)
  • 0304-8608
  • 1432-8798
OpenAlex
W2005517242
Cited by
44
References
15
Citations by year
2023: 2 · 2021: 6 · 2020: 8 · 2014: 1 · 2012: 2
FWCI
1.43
Type
journal article
Language
en
Open access
Locations
  • Archives of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
infectious bronchitis virus, viral morphogenesis, phagocytosis, micropinocytosis, viral budding, endoplasmic reticulum, cytoplasmic vacuoles, cell lysis, virion release, membrane fusion
MeSH terms
Animals, Infectious bronchitis virus, Cell Membrane, Chickens, Coronaviridae, Endoplasmic Reticulum, Kidney, Morphogenesis, Phagocytosis, Pinocytosis, Vacuoles, Virus Replication, Culture Techniques

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