Pyruvate blocks expression of sensitivity to antimycin A and chloramphenicol
Abstract
Selectivity in Chinese hamster cells with antimycin A and chloramphenicol depends on a metabolic balance which can be modulated by varying the level of exogenous pyruvate. The effects of both inhibitors are most clearly seen in pyruvate-free nutrients. Addition of 1 mM pyruvate in plating assays shifts dose-response curves for antimycin A or chloramphenicol to higher concentration levels and reduces the differential in response between sensitive and resistant cells. In mass populations, growth inhibition by antimycin A is reduced by adding pyruvate, and growth curves for sensitive and resistant cells tend to converge. These observations show that responses to antimitochondrial drugs can be conditioned by extrinsic factors and indicate the need for further definition of selective systems.
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Inhibitors of cellular respiration block proliferation, suggesting that most cancer cells require respiration to proliferate.
“Moreover, inhibitors of cellular respiration block proliferation, suggesting that most cancer cells require respiration in order to proliferate (Harris, 1980; Howell and Sager, 1979; Kroll et al., 1983; Loffer and Schneider, 1982; Zhang et al., 2014b).”
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- Authors
- Harris, M.
- Affiliations
- University of California, Berkeley (US)
- Corresponding author
- Harris, Morgan
- ORCID
- Harris, Morgan
- Venue
- Somatic Cell Genetics
- Published
- 1980-11
- Volume
- 6
- Issue
- 6
- Pages
- 699-708
- ISSN
- 0098-0366 (ISSN-L)
- 1572-9931
- OpenAlex
- W2043284251
- Cited by
- 30
- References
- 23
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 2
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Somatic Cell and Molecular Genetics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- pyruvate, antimycin A, chloramphenicol, drug sensitivity, drug resistance, Chinese hamster cells, mitochondrial inhibitors, metabolic balance, dose-response relationship, growth inhibition, selective toxicity
- MeSH terms
- Animals, Antimycin A, Cell Division, Cell Line, Chloramphenicol, Cricetulus, Culture Media, Dose-Response Relationship, Drug, Cricetinae, Lung, Mice, Inbred BALB C, Pyruvates, Mice
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