Cited by 30Mitochondrial Function and PathologyCancer, Hypoxia, and MetabolismBacterial Genetics and Biotechnology

Pyruvate blocks expression of sensitivity to antimycin A and chloramphenicol

Harris, Morgan·1980·Somatic Cell Genetics

Abstract

Selectivity in Chinese hamster cells with antimycin A and chloramphenicol depends on a metabolic balance which can be modulated by varying the level of exogenous pyruvate. The effects of both inhibitors are most clearly seen in pyruvate-free nutrients. Addition of 1 mM pyruvate in plating assays shifts dose-response curves for antimycin A or chloramphenicol to higher concentration levels and reduces the differential in response between sensitive and resistant cells. In mass populations, growth inhibition by antimycin A is reduced by adding pyruvate, and growth curves for sensitive and resistant cells tend to converge. These observations show that responses to antimitochondrial drugs can be conditioned by extrinsic factors and indicate the need for further definition of selective systems.

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Crossref · 10.1007/bf01538969

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  • Inhibitors of cellular respiration block proliferation, suggesting that most cancer cells require respiration to proliferate.

    “Moreover, inhibitors of cellular respiration block proliferation, suggesting that most cancer cells require respiration in order to proliferate (Harris, 1980; Howell and Sager, 1979; Kroll et al., 1983; Loffer and Schneider, 1982; Zhang et al., 2014b).”

    From Targeting Metabolism for Cancer Therapy (2017) · cites this paper as Harris, 1980

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Authors
Harris, M.
Affiliations
University of California, Berkeley (US)
Corresponding author
Harris, Morgan
Venue
Somatic Cell Genetics
Published
1980-11
Volume
6
Issue
6
Pages
699-708
ISSN
  • 0098-0366 (ISSN-L)
  • 1572-9931
OpenAlex
W2043284251
Cited by
30
References
23
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 2
FWCI
1.10
Type
journal article
Language
en
Open access
closed
Locations
  • Somatic Cell and Molecular Genetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Cancer Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
pyruvate, antimycin A, chloramphenicol, drug sensitivity, drug resistance, Chinese hamster cells, mitochondrial inhibitors, metabolic balance, dose-response relationship, growth inhibition, selective toxicity
MeSH terms
Animals, Antimycin A, Cell Division, Cell Line, Chloramphenicol, Cricetulus, Culture Media, Dose-Response Relationship, Drug, Cricetinae, Lung, Mice, Inbred BALB C, Pyruvates, Mice

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