Transformation of selenate and selenite to elemental selenium byDesulfovibrio desulfuricans
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Desulfovibrio desulfuricans (DSM 1924) can be adapted to grow in the presence of 10 mM selenate or 0.1 mM selenite. This growth occurred in media containing formate as the electron donor and either fumarate or sulfate as the electron acceptor. As determined by electron microscopy with energy-dispersive X-ray analysis, selenate and selenite were reduced to elemental selenium which accumulated inside the cells. Selenium granules resulting from selenite metabolism were cytoplasmic while granules of selenium resulting from selenate reduction appeared to be in the periplasmic region. The accumulation of red elemental selenium in the media following stationary phase resulted from cell lysis with the liberation of selenium granules. Growth did not occur with either selenate or selenite as the electron acceptor and13C nuclear magnetic resonance indicated that neither selenium oxyanion interfered with fumarate respiration. At 1 μM selenate and 100 μM selenite, reduction byD. desulfuricans was 95% and 97%, respectively. The high level of total selenate and selenite reduced indicated the suitability ofD. desulfuricans for selenium detoxification.
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Organisms known to reduce Se(VI) enzymatically include W. succinogenes, D. desulfuricans, Pseudomonas stutzeri, E. cloacae and E. coli.
“Organisms that are known to reduce Se(VI) enzymatically include W. succinogenes [104], D. desulfuricans [105], Pseudomonas stutzeri [106], E. cloacae [107] and E. coli [108].”
From Microbial reduction of metals and radionuclides (2003) · cites this paper as [105]
The Discussion (page not provided) reports that D. desulfuricans reduces selenate to elemental selenium and attributes the reduction to cellular activity rather than a chemical reaction in the growth medium. It does not establish that the reduction is enzymatic, so that qualifier is not fully supported. Citation [105] is attached only to D. desulfuricans, not to the other organisms listed. (LLM, conf=0.83)
Experimental — accuracy not yet measuredIn these examples, Se(VI) reduction does not support growth, and seems to be incidental to the physiology of the organism.
“In these examples, Se(VI) reduction does not support growth, and seems to be incidental to the physiology of the organism.”
From Microbial reduction of metals and radionuclides (2003) · cites this paper as [105]
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- Authors
- Tomei, F. A., Barton, L. L., Lemanski, C. L., Zocco, T. G., Fink, N. H., & Sillerud, L. O.
- Affiliations
- Georgia Institute of Technology (US)
- University of New Mexico (US)
- Los Alamos National Laboratory (US)
- Venue
- Journal of Industrial Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-03
- Volume
- 14
- Issue
- 3-4
- Pages
- 329-336
- ISSN
- 1367-5435 (ISSN-L)
- 0169-4146
- 1476-5535
- OpenAlex
- W2085459704
- Cited by
- 148
- References
- 37
- Citations by year
- 2025: 3 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 8 · 2020: 8 · 2019: 2 · 2018: 6 · 2017: 7 · 2016: 8 · 2015: 6 · 2014: 7 · 2013: 5 · 2012: 4
- FWCI
- 4.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Industrial Microbiology & Biotechnology (publishedVersion)
- Funders
- U.S. Department of Energy
- Los Alamos National Laboratory
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Mine drainage and remediation techniques
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Physical Sciences › Engineering › Biomedical Engineering › Metal Extraction and Bioleaching
- Keywords
- Desulfovibrio desulfuricans, selenate reduction, selenite reduction, elemental selenium, selenium detoxification, fumarate respiration, sulfate respiration, cell lysis, selenium oxyanions
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