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Cited by 241

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Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids

Rouser, George, Kritchevsky, Gene, Simon, Gerald, Nelson, Gary J.·1967·Lipids

Abstract

Abstracts: Quantitative elution of acidic and neutral glycolipids of brain and spinach leaves from silicic acid columns with acetone was demonstrated. Cerebrosides and sulfatides of brain and sulfolipid and glycosyl diglycerides of spinach leaves were eluted quantitatively with acetone while prospholipids remained on the column. The observations provide the basis for an analytical procedure employing column and quantitative thin‐layer chromatography (TLC). Sephadex column chromatography is utilized for separation of lipids from nonlipids; silicic acid column chromatography for separation into neutral lipid, glycolipid and phospholipid fractions; and quantitative TLC for analysis of lipid classes of each column fraction.

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Metadata

Authors
Rouser, G., Kritchevsky, G., Simon, G., & Nelson, G. J.
Venue
Lipids
Publisher
Wiley
Published
1967-01
Volume
2
Issue
1
Pages
37-40
ISSN
  • 0024-4201 (ISSN-L)
  • 1558-9307
OpenAlex
W2046021520
Cited by
241
References
0
FWCI
4.56
Type
journal article
Language
en
Open access
closed

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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