Utilization of organic substrates during mixotrophic and heterotrophic cultivation of algae
Abstract
Pure cultures of Chlorella pyrenoidosa (82) and Scenedesmus obliquus (125) were grown in the nutrient medium according to Benson in the presence of 0·05 m sugars or 0·025 m sodium salts of organic acids. The density of culture was measured throughout the course of growth. Satisfactory heterotrophic sources of nutrition for Chlorella pyrenoidosa appear to be galactose, glucose and acetate, whereas Scenedesmus utilizes glucose, cellobiose and acetate. The growth of Chlorella in the light is enhanced by galactose, glucose, fructose, cellobiose and maltose, that of Scenedesmus by glucose, fructose, cellobiose, galactose, maltose, acetate and pyruvate. Soluble starch suppresses growth of both cultures. The role of the substrates is discussed. It follows from the results that the growth-promoting sugars and organic acids can act not only as a source of carbon during general carbon shortage but also as ergastic material. The mechanism of utilization of some organic substrates will be taken up in a subsequent paper.
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Monosaccharides had more significant growth-promoting effect on C. pyrenoidosa than disaccharides at different light intensities (1080 and 1800 lux); among hexoses, galactose gave the highest mixotrophic cell growth, followed by glucose and fructose.
“A different study found that monosaccharides had more significant growth-promoting effect on C. pyrenoidosa than disaccharides (cellobiose, maltose and sucrose) at different light intensities (1080 and 1800 lux); and, among the hexoses, galactose gave the highest mixotrophic cell growth, followed by glucose and fructose [70].”
From Challenges and Opportunities to Increase Economic Feasibility and Sustainability of Mixotrophic Cultivation of Green Microalgae of the Genus Chlorella (2022) · cites this paper as [70]
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- Authors
- Dvořáková-Hladká, J.
- Affiliations
- Czech Academy of Sciences (CZ)
- Corresponding author
- Dvořáková-Hladká, J.
- Venue
- Biologia Plantarum
- Publisher
- Institute of Experimental Botany
- Published
- 1966-09
- Volume
- 8
- Issue
- 5
- Pages
- 354-361
- ISSN
- 0006-3134 (ISSN-L)
- 1573-8264
- OpenAlex
- W2326316376
- Cited by
- 25
- References
- 21
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2012: 1
- FWCI
- 2.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologia Plantarum (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Keywords
- Chlorella pyrenoidosa, Scenedesmus obliquus, mixotrophic cultivation, heterotrophic cultivation, organic substrates, sugar, organic acids, acetate, galactose, glucose, cellobiose, soluble starch, algal growth, carbon source
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