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Cited by 46Thyroid Disorders and TreatmentsClinical Laboratory Practices and Quality ControlGrowth Hormone and Insulin-like Growth Factors

Measurement of cAMP accumulation in Chinese hamster ovary cells transfected with the recombinant human TSH receptor (CHO-R): a new bioassay for human thyrotropin

Persani, L., Tonacchera, M., Beck-Peccoz, P., Vitti, P., Mammoli, C., Chiovato, L., Elisei, R., Faglia, G. +3 more·1993·Journal of Endocrinological Investigation
DOI 10.1007/bf03348894
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Abstract

Circulating TSH bioactivity may vary in several clinical and experimental conditions. Since the reliability of the current methods for the measurement of TSH bioactivity is limited, a new bioassay based on cAMP accumulation in Chinese Hamster Ovary cells transfected with recombinant human TSH receptor (CHO-R) was set up. The sensitivity was 0.3 +/- 0.1, 0.4 +/- 0.1 and 0.01 +/- 0.01 micrograms/L for TSH IRP 80/558, recombinant human TSH and bovine TSH, respectively. Standard curves were parallel, and the intra- and inter-assay coefficients of variation were 13 +/- 1.1% and 22 +/- 1.9%, respectively. LH, FSH, CG and TSH subunits did not stimulate cAMP accumulation up to high concentrations. Circulating TSH was partially purified by immunoaffinity separation and concentrated before being bioassayed. However, plain sera with high TSH levels, such as those from primary hypothyroid patients (PH), could be directly tested in CHO-R bioassay, provided that sera were added at concentrations lower than 10%. TSH from 6 normal subjects had biological to immunological ratio (B/I) ranging from 0.6 to 2.1 (mean +/- SD = 1.4 +/- 0.5). TSH from 6 patients with PH showed bioactivity significantly lower than in normals (B/I = 0.6 +/- 0.3; p < 0.001; range = 0.3-1.1). TSH from 5 patients with central hypothyroidism of hypothalamic origin (CH) had undetectable basal bioactivity (B/I < 0.2), which normalized in only one patient after acute TRH and in all patients after chronic TRH administration. In conclusion, CHO-R cells provide an excellent tool for evaluating TSH bioactivity, owing to high sensitivity, specificity, reproducibility and feasibility of the assay.(ABSTRACT TRUNCATED AT 250 WORDS)

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Crossref · 10.1007/bf03348894

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  • Poor intrinsic biological activity of secreted TSH molecules is a pathological mechanism accounting for central hypothyroidism.

    “(c) poor intrinsic biological activity of secreted TSH molecules [14-18].”

    From 2018 European Thyroid Association (ETA) Guidelines on the Diagnosis and Management of Central Hypothyroidism (2018) · cites this paper as [18]

  • Impaired bioactivity of circulating TSH has been prevalently demonstrated by in vitro bioassays, but can also be supported in vivo by impaired increment of free T4 and/or T3 following TSH response upon TRH stimulation test.

    “The impaired bioactivity of circulating TSH has been prevalently demonstrated by in vitro bioassays (15), but this phenomenon can also be supported in vivo by the impaired increment of circulating free T4 and/or T3 following the TSH response upon TRH stimulation test (18, 19).”

    From The diagnosis and management of central hypothyroidism in 2018 (2019) · cites this paper as [15]

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Authors
Persani, L., Tonacchera, M., Beck-Peccoz, P., Vitti, P., Mammoli, C., Chiovato, L., Elisei, R., Faglia, G., Ludgate, M., Vassart, G., & Pinchera, A.
Affiliations
  • University of Milan (IT)
  • University of Pisa (IT)
  • Université Libre de Bruxelles (BE)
ORCID
Venue
Journal of Endocrinological Investigation
Publisher
Springer Science and Business Media LLC
Published
1993-07
Volume
16
Issue
7
Pages
511-519
ISSN
  • 0391-4097 (ISSN-L)
  • 1720-8386
DOI
10.1007/bf03348894
OpenAlex
W2077223663
Cited by
45
References
34
Citations by year
2021: 2 · 2019: 3 · 2018: 1 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 1
FWCI
1.17
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Endocrinological Investigation (publishedVersion)
  • PubMed (publishedVersion)
  • Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
thyroid-stimulating hormone, cAMP accumulation, primary hypothyroidism, central hypothyroidism, thyrotropin-releasing hormone
MeSH terms
Cyclic AMP, Adult, Aged, Animals, Biological Assay, Female, Cricetinae, Humans, Hypothyroidism, Male, Middle Aged, Receptors, Thyrotropin, Sensitivity and Specificity, Thyrotropin, Transfection, CHO Cells

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